Team:Princeton/Experiments

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PRINCETON IGEM 2008

Home Project Overview Project Details Experiments Results Notebook
Parts Submitted to the Registry Modeling The Team Gallery




Bio-brick Creation

Lab Protocols

1. Primer Design

2. PCR Amplification

3. PCR SOEing

4. Run Gel

5. Gel Extraction

6. Digestion

8. CIP Treatment

9. PCR Purification

10. Ligation

11. Transformation and Plating

12. Extract DNA (Miniprep)

13. Restriction Map/ Digest

14. Re-transform with selected plasmid

15. Extract DNA (Maxiprep or Midiprep)

16. Sequence

Bio-Brick Verification

Cell Culture Process

1. Lentivirus Production

2. Lentivirus Harvesting

3. Infection


Also,

1. Transfection (nonviral)

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