Team:Paris/Modeling/f0

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Method & Algorithm : ƒ0


Specific Plasmid Characterisation for ƒ0
In the exponential phase of growth, at the steady state, we have

EqF0.jpg

param signification unit value comments
[exprJ23101] expression rate of
J23101 with RBS E0032 		nM.min-1
γGFP dilution-degradation rate
of GFP(mut3b) 		min-1
[GFP] GFP concentration at steady-state nM
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration 		nM.au-1 (1/79.429)

That will directly give us ƒ0

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