Team:Paris/Modeling/f0

From 2008.igem.org

Revision as of 08:46, 29 October 2008 by Hugo (Talk | contribs)

Method & Algorithm : ƒ0


Specific Plasmid Characterisation for ƒ0
In the exponential phase of growth, at the steady state, we have

EqF0.jpg

↓ Prior for Characterization ↑


param signification unit value comments
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration
↓ gives ↓ 		nM.au-1 (1/79.429)
[GFP] GFP concentration at steady-state nM
γGFP dilution-degradation rate
of GFP(mut3b)
↓ gives ↓ 		min-1 0.0198 Only dilution :
Time Cell Division : 35 min.
ƒ0 activity of
J23101 with RBS E0032 		nM.min-1


That will directly give us ƒ0
Retrieved from "http://2008.igem.org/Team:Paris/Modeling/f0"