Virginia/8 July 2008

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Goals

  • Prepare overnight broth of successful single colony of Promoter + RBS transformation
  • Plate Screening plasmid (psb1a10) when it arrives from iGem
  • Try again with ligation of RFP and GFP enzyme cut DNA
  • Transform this ligation and pray that it grows in the incubator
  • Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
  • Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon


Notes

  • Maxiprepped bba_B0015 and bba_B0034 smell strongly of alcohol, we are dubious of the content of the prepared DNA


Notebook

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