Team:KULeuven/Primers
From 2008.igem.org
dock/undock dropdown
Table of Oligos | |||||
---|---|---|---|---|---|
Number | Name | Sequence | Length | Comments | |
1 | T7 Fw, no tag | catcatgaattcgcggccgcttctagatgaacacgattaacatcgc | 46bp | {{{Tm}}} | Forward T7 RNApol primer, without UmuD tag |
2 | T7 Rev | ctgcagcggccgctactagtattattacgcgaacgcgaagtccg | 44bp | {{{Tm}}} | Reverse T7 RNApol primer |
3 | T7 Fw, UmuD tag, part 1 | ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc | 62bp | {{{Tm}}} | Forward T7 RNApol primer, with UmuD tag, part 1 |
4 | T7 Tw, UmuD tag, part 2 | catcatgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag | 95bp | {{{Tm}}} | Forward T7 RNApol primer, with UmuD tag, part 2, very expensive |
5 | GFP-LVA Fw | catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg | 55bp | {{{Tm}}} | Forward GFP-LVA primer |
6 | GFP-LVA Rev | ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc | 64bp | {{{Tm}}} | Reverse GFP-LVA primer |
7 | VF2 | TGCCACCTGACGTCTAAGAA | 20bp | {{{Tm}}} | Forward primer for sequence analysis |
8 | VR | ATTACCGCCTTTGAGTGAGC | 20bp | {{{Tm}}} | Reverse primer for sequence analysis |