Team:KULeuven/Primers

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Table of Oligos
Number Name Sequence Length Comments
1 T7 Fw, no tag catcatgaattcgcggccgcttctagatgaacacgattaacatcgc 46bp {{{Tm}}} Forward T7 RNApol primer, without UmuD tag
2 T7 Rev ctgcagcggccgctactagtattattacgcgaacgcgaagtccg 44bp {{{Tm}}} Reverse T7 RNApol primer
3 T7 Fw, UmuD tag, part 1 ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc 62bp {{{Tm}}} Forward T7 RNApol primer, with UmuD tag, part 1
4 T7 Tw, UmuD tag, part 2 catcatgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag 95bp {{{Tm}}} Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
5 GFP-LVA Fw catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg 55bp {{{Tm}}} Forward GFP-LVA primer
6 GFP-LVA Rev ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc 64bp {{{Tm}}} Reverse GFP-LVA primer
7 VF2 TGCCACCTGACGTCTAAGAA 20bp {{{Tm}}} Forward primer for sequence analysis
8 VR ATTACCGCCTTTGAGTGAGC 20bp {{{Tm}}} Reverse primer for sequence analysis