Team:KULeuven/Primers

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Table of Oligos
Number Name Sequence Length Comments
1076 T7 Fw, no tag atgaattcgcggccgcttctagatgaacacgattaacatcgc 42bp {{{Tm}}} Forward T7 RNApol primer, without UmuD tag
1077 T7 Rev ctgcagcggccgctactagtattattacgcgaacgcgaagtccg 44bp {{{Tm}}} Reverse T7 RNApol primer
1078 T7 Fw, UmuD tag, part 1 ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc 62bp {{{Tm}}} Forward T7 RNApol primer, with UmuD tag, part 1
1079 T7 Tw, UmuD tag, part 2 atgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag 91bp {{{Tm}}} Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
1084 GFP-LVA Fw catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg 55bp {{{Tm}}} Forward GFP-LVA primer
1085 GFP-LVA Rev ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc 64bp {{{Tm}}} Reverse GFP-LVA primer
1074 VF2 TGCCACCTGACGTCTAAGAA 20bp {{{Tm}}} Forward primer for sequence analysis
1075 VR ATTACCGCCTTTGAGTGAGC 20bp {{{Tm}}} Reverse primer for sequence analysis