Team:KULeuven/Primers
From 2008.igem.org
dock/undock dropdown
Table of Oligos | |||||
---|---|---|---|---|---|
Number | Name | Sequence | Length | Comments | |
1076 | T7 Fw, no tag | atgaattcgcggccgcttctagatgaacacgattaacatcgc | 42bp | {{{Tm}}} | Forward T7 RNApol primer, without UmuD tag |
1077 | T7 Rev | ctgcagcggccgctactagtattattacgcgaacgcgaagtccg | 44bp | {{{Tm}}} | Reverse T7 RNApol primer |
1078 | T7 Fw, UmuD tag, part 1 | ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc | 62bp | {{{Tm}}} | Forward T7 RNApol primer, with UmuD tag, part 1 |
1079 | T7 Tw, UmuD tag, part 2 | atgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag | 91bp | {{{Tm}}} | Forward T7 RNApol primer, with UmuD tag, part 2, very expensive |
1084 | GFP-LVA Fw | catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg | 55bp | {{{Tm}}} | Forward GFP-LVA primer |
1085 | GFP-LVA Rev | ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc | 64bp | {{{Tm}}} | Reverse GFP-LVA primer |
1074 | VF2 | TGCCACCTGACGTCTAAGAA | 20bp | {{{Tm}}} | Forward primer for sequence analysis |
1075 | VR | ATTACCGCCTTTGAGTGAGC | 20bp | {{{Tm}}} | Reverse primer for sequence analysis |