Wisconsin: Lignin Project/10 July 2008
From 2008.igem.org
Team Sorbitol
Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.
Started the following cultures:
DH5a-PBAD30 : to harvest the plasmid to clone out the srl operon DH5a-pBAD30-srlD: from colony 2 to freeze down for back up DH5a-pBAD18 - to insert srld and the operon into.
MG1655, JW3890, RL257 - all to make chemically competent