Team:KULeuven/Primers

Revision as of 14:18, 31 July 2008 by Bmoeyaert (Talk | contribs)

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Table of Oligos
Number	Name	Sequence	Length	Comments
1076	T7 Fw, no tag	ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC	42bp	{{{Tm}}}	Forward T7 RNApol primer, without UmuD tag
1077	T7 Rev	CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG	44bp	{{{Tm}}}	Reverse T7 RNApol primer
1078	T7 Fw, UmuD tag, part 1	CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC	62bp	{{{Tm}}}	Forward T7 RNApol primer, with UmuD tag, part 1
1079	T7 Tw, UmuD tag, part 2	ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G	91bp	{{{Tm}}}	Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
1084	GFP-LVA Fw	CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG	55bp	{{{Tm}}}	Forward GFP-LVA primer
1085	GFP-LVA Rev	CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC	64bp	{{{Tm}}}	Reverse GFP-LVA primer
1074	VF2	TGCCACCTGA CGTCTAAGAA	20bp	{{{Tm}}}	Forward primer for sequence analysis
1075	VR	ATTACCGCCT TTGAGTGAGC	20bp	{{{Tm}}}	Reverse primer for sequence analysis