Edinburgh/11 July 2008

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Edinburgh iGEM 2008

 

Week 4

Friday 11 July 08

  • Submitted M19, M21 and M22 for sequencing using primer pTG262f1, to check for clean removal of the EcoRI site.
  • Revived Cellulomonas fimi in NB and plated to NA and NA+glucose. Plates 28 and 29 incubated at 30°C.
  • ZY and XH repeated rbs+dxs ligation (L11).
  • Transformation of JM109 with L9 (pSB1A2+crtB )and L10 (pSB1A2+crtI-mut). Plates 30 to 33.
  • possible rbs+dxs clones from un-numbered plates were streaked to plate 34.
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