From 2008.igem.org

Revision as of 03:09, 27 August 2008 by Gracek (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Birds Eye View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
	Notebook (t)	Meetings (t)

Things we did today

Wetlab work

Ran RE digests on gel

Grace

Gel did not resolve. Bands were convex, poor separation between bands. Bad buffer?
Redid RE digests from yesterday

Used PCR products of nir and J33207

Inoculated for plasmid prep

Grace

BB-pRL1383a and J33207 in TB+amp₁₀₀

Gel Purified

Krystle

Ran overnight ligation products on a 3% agarose gel

discussion: If we decide to do the 3A in two parts, we will have to re-restriction digest the first part before moving on

Transformation of ligated GFPf + TT

Krystle<strong>

Performed transformation protocol with DB3.1 and GFPf + tt ligation from 08/25

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

Team:Hawaii/Notebook/2008-08-26