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Team:KULeuven/Project/Reset
From 2008.igem.org
Contents |
Reset
BioBricks
Components
The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 BBa_I712074]) followed by a RiboLock ([http://partsregistry.org/Part:BBa_J23078 BBa_J23078]). The aiiA gene ([http://partsregistry.org/Part:BBa_C0060 BBa_C0060]), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. This inactivates the 3OC6HSL autoinducer molecule, efficiently removing it from the active system. ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference])
Action
The reset mechanism is made to destroy all HSL at the time, when significant input re-emerges. Originally, a pulse of lactonase was thought to do the thing, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the concentration should be high enough and the device should work.
The aiiA gene coding for lactonase is regulated by the output signal from the filter, so that background input signal does not result in timer reset.
Input
Output
Filter
InverTimer
Reset
Cell Death
Memory
