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Team:KULeuven/Project/Reset
From 2008.igem.org
Contents |
Reset
BioBricks
Components
The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 BBa_I712074]) followed by a RiboLock ([http://partsregistry.org/Part:BBa_J23078 BBa_J23078]). The aiiA gene ([http://partsregistry.org/Part:BBa_C0060 BBa_C0060]), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference])
Action
This device was conceived to reset the Timer.
When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. In this way, the timer is reset and the cell may live to see another day.
Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised that is described here. Now the the amount of lactonase produced should be high enough for the reset to function properly.
Input
Output
Filter
InverTimer
Reset
Cell Death
Memory
