Team:KULeuven/Ligation

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• The Team
  Research Labs and Groups Students Instructors Advisors Pictures
• The Project
  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

  Concerns & Issues

  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

  Afterword References
• Submitted Parts
  Sandbox
• Modeling
  Overview Kinetic Constants Components

  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
• Data Analysis
  Overview New Parts

  GFP (LVA-tag) Other

  Components

  Input Output Other
• Software
  Multi-cell Toolbox Simbiology2LaTeX Toolbox
• Notebook
  Protocols Reports

  Daily Weekly

  Lab Data

  Freezer Primers Ligation

  Tools Press Acknowledgments

New Parts

BioBrick	Description	Status	Experience
[http://partsregistry.org/Part:BBa_K145001 K145001]	T7 DNA polymerase (no tag)
[http://partsregistry.org/Part:BBa_K145013 K145013]	antisense LuxI
[http://partsregistry.org/Part:BBa_K145014 K145014]	T7 DNA polymerase with UmuD tag
[http://partsregistry.org/Part:BBa_K145015 K145015]	GFP with LVA tag
[http://partsregistry.org/Part:BBa_K145150 K145150]	hybrid promoter
[http://partsregistry.org/Part:BBa_K145151 K145151]	ccdB coding region

New Devices

BioBrick	Components	Description	Status	Experience
[http://partsregistry.org/Part:BBa_K145201 K145201]	J23116+B0032+C0040+B0015	Input
[http://partsregistry.org/Part:BBa_K145205 K145205]	R0040+B0032+K145015+B0015	Output (LVA tag)
[http://partsregistry.org/Part:BBa_K145252 K145252]	R0040+E0240	Output (no tag)
[http://partsregistry.org/Part:BBa_K145280 K145280]	K145201+K145205	Input-Output: test	electroporation
[http://partsregistry.org/Part:BBa_K145279 K145279]	K145201+K145252	Input-Output: test	electroporation
[http://partsregistry.org/Part:BBa_K145258 K145258]	R0040+B0033+C0056+B0015	Memory - part 1	electroporation
[http://partsregistry.org/Part:BBa_K145259 K145259]	R0053+P0152+R1052+P0353	Memory - part 2
[http://partsregistry.org/Part:BBa_K145210 K145210]	K145258+K145259	Memory
[http://partsregistry.org/Part:BBa_K145211 K145211]	R1052+K145013+B0015	Memory - part 3
[http://partsregistry.org/Part:BBa_K145215 K145215]	R0040+J23066	Filter - part 1
[http://partsregistry.org/Part:BBa_K145278 K145278]	J23109+J23032+K145001+B0015	Filter (old) - part 2	electroporation
[http://partsregistry.org/Part:BBa_K145277 K145277]	J23109+J23078+K145001+B0015	Filter (new) - part 2
[http://partsregistry.org/Part:BBa_K145275 K145275]	K145215+K145278	Filter (old)
[http://partsregistry.org/Part:BBa_K145276 K145276]	K145215+K145277	Filter (new)
[http://partsregistry.org/Part:BBa_K145253 K145253]	I712074+J23032+C0012+B0015	Invertimer (old) - part 1
[http://partsregistry.org/Part:BBa_K145255 K145255]	I712074+J23078+C0012+B0015	Invertimer (new) - part 1
[http://partsregistry.org/Part:BBa_K145254 K145254]	R0011+B0032+C0061+B0015	Invertimer - part 2
[http://partsregistry.org/Part:BBa_K145220 K145220]	K145255+K145254	Invertimer (new)
[http://partsregistry.org/Part:BBa_K145104 K145104]	K145253+K145254	Invertimer (old)
[http://partsregistry.org/Part:BBa_K145251 K145251]	I712074+J23032+C0060+B0015	Reset (old)
[http://partsregistry.org/Part:BBa_K145225 K145225]	I712074+J23078+C0060+B0015	Reset (new)
[http://partsregistry.org/Part:BBa_K145256 K145256]	K145150+B0034+C0062	Cell Death - part 1
[http://partsregistry.org/Part:BBa_K145257 K145257]	B0014+B0033+K145151+B0015	Cell Death - part 2
[http://partsregistry.org/Part:BBa_K145230 K145230]	K145256+K145257	Cell Death
[http://partsregistry.org/Part:BBa_K145273 K145273]	R0040+P0353	test K145150 - part 2
[http://partsregistry.org/Part:BBa_K145270 K145270]	J23116+B0032+C0062+B0015	test K145150 - part 3	electroporation
[http://partsregistry.org/Part:BBa_K145269 K145269]	K145150+E0240	test K145150 - part 4
[http://partsregistry.org/Part:BBa_K145268 K145268]	K145201+K145273	test K145150 - part 5
[http://partsregistry.org/Part:BBa_K145267 K145267]	K145270+K145269	test K145150 - part 6
[http://partsregistry.org/Part:BBa_K145266 K145266]	K145268+K145267	test K145150
[http://partsregistry.org/Part:BBa_K145271 K145271]	I712074+J23078+E0040+B0015	test filter (new) - part 3
[http://partsregistry.org/Part:BBa_K145272 K145272]	I712074+J23032+E0040+B0015	test filter (old) - part 3
[http://partsregistry.org/Part:BBa_K145265 K145265]	K145275+K145272	test filter (old)
[http://partsregistry.org/Part:BBa_K145264 K145264]	K145276+K145271	test filter (new)

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