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Team:Chiba/protocol
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Revision as of 02:43, 11 September 2008 by
Hiroki
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Notebook
Contents
1
Transformation
1.1
Zymo comp.
1.2
Electro poration
2
DNA Purification
2.1
Sigma prep
2.2
Qiaprep
2.3
Zymo cleam
3
Agarose gel electrophoresis
4
Digestion
5
PCR
6
Ligation
6.1
Gel extract
6.2
Dephosphorylation of DNA
6.3
Phosphorylation of DNA
6.4
Ligation
Transformation
Zymo comp.
Electro poration
DNA Purification
Sigma prep
Qiaprep
Zymo cleam
Agarose gel electrophoresis
Digestion
PCR
Ligation
Gel extract
Dephosphorylation of DNA
Phosphorylation of DNA
Ligation
"
