Team:Hawaii/Notebook/2008-09-26

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Revision as of 20:16, 26 September 2008 by Gracek (Talk | contribs)
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Things we did today

Wetlab work

Verification of Transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1.5 hours. One microliter of PCR product were loaded into each well.
Construct Colonies
nir+rbs+GFP + tt 3
nir + rbs+GFP+tt1 1
plac + rbs+GFP+tt1 1
nir + rbs+GFP+tt2 5
plac + rbs+GFP+tt2 0
pSB1A3 to self (- control) 0
J33207 + pRL1383a (plated on IPTG+Xgal)
J33207 + pRL1383a (1:10) (plated on Xgal)
pRL1383a to self (+ control)
J33207 plasmid (+ control, plated on IPTG+Xgal) 162 (all blue!)
pRL1383a plasmid (+ control)
no DNA 0
  • Colony PCR of transformants

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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