Alberta NINT/2 July 2008
From 2008.igem.org
lab work (SD):
Extracted DNA from gel fragments (~600 bp) for K102002 and K102008 digested with EcoRI + PstI. Ligated K102002 into pSB4A5 and K102008 into pSB4A5 (using pSB4A5 as a negative control). Transformed XL1-B cells with these ligations.