Team:Hawaii/Notebook/2008-06-12

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Things we did today

Wetlab work

Triparental conjugation

Grace
  • Began triparental conjugation between DH5α transformed with pRL1383a, E. coli with RP1, and PCC6803 (see experiment)
  • Innoculated BG-11 + 5% LB plates

Purified BBa_B0034 colonies

Krystle
  • Streaked out colonies onto fresh LB+amp100 plates

Preparation for making competent cells

Krystle & Grace
  • Autoclaved detergent-free erlenmeyer flasks
  • Innoculated 250 ml SOB w/ DH5α cryostock; shook overnight at 26C with shaking (150 rpm)

Made BG-11+sm+sp plates

Krystle
  • Made 20 plates
  • 2.5 μg/ml each of sm and sp

Made SOB media

Krystle
  • 2 batches

Made BG-11

Norman

Drylab Work

Sequence synthesis

Grace
  • rbc promoter Biobrick sequence to be synthesized

Parts list

Norman
  • Parts list submitted by team members; Norman will send a request to iGEM for parts not on filter paper

Discussion

Quote of the Day

  • If our action is not recorded, we are doomed to either repeat the error, or fail to replicate the success.
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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