From 2008.igem.org

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Things we did today

Wetlab work

Insertion of lac-GFP device into BB-pRL1383a

Grace

EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.

• Ran RE digests on gel

• quantification of bands

• Extracted bands from gel
• Ligated J04430 and BB-pRL1383a
• Used 5μl to transform DH5α

Antibiotic test for BB-pRL1383a

Grace

• Made LB+sp_variable plates with 40 μl 20X X-gal
• Transformed 100 μl DH5α with 2 &mul; BB-pRL1383a ng
• Plated 50 &m;ul cells + SOB on each plate

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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