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From 2008.igem.org

HOME	PROJECT	TEAM	SUBMITTED PARTS	MODELING	WETLAB	LAB-BOOK	BIOSAFETY AND PROTOCOLS

Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.

Contents 1 Protocols 2 Week 1: from 07/21/08 to 07/27/08 3 Week 2: from 07/28/08 to 08/03/08 4 Week 3: from 08/04/08 to 08/10/08

Protocols

Protocol	Biosafety level
Plates preparation	1
Amplification	1
Transformation	1
Inoculation	1
Miniprep	1
Digestion reaction	1
Gel preparation	2
Electrophoretic run	2
Gel extraction	2
Ligation reaction	1
Chemiocompetent cells	1
Mediums and buffers	1
Antibiotics stocks preparation	1
IPTG stocks preparation	1
Fluorescence test	1
M9 supplemented media	1

Week 1: from 07/21/08 to 07/27/08

1. Enzymatic digestion of: C0051 E\S, LACI* X\P.
2. Gel preparation , electrophoretic run.
3. Amplification of: TETR , PUV, PRECA.

Week 2: from 07/28/08 to 08/03/08

1. Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
   
2. Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240
   

Week 3: from 08/04/08 to 08/10/08

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