"
Team:NTU-Singapore/Notebook/30 June 2008
From 2008.igem.org
Monday 30 June:
Chin Chong
- Stock check of items purchased so far and shifted them into new lab with Darius's help
- Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed the purchase and sequence should arrive in 2-4 weeks
- Purchased items from SBS general store
- Did inoculation of colonies from Top cells containing:
- LacI-GFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004],
- Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]
- GFP reporter devices with Weak [http://partsregistry.org/Part:BBa_J23150 BBa_J23150],
- Medium [http://partsregistry.org/Part:BBa_J23151 BBa_J23151] and
- Strong [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] promoters
- Incubate cells in 5ml of LB broth containing the respective anitbiotics and allow them to grow overnight
- This is the slight modification from the standard protocol suggested in the Newsletter Vol.1 which used supplemented M9 medium instead of LB broth
- M9 medium with glycerol will be introduced in the following day after the cells have been allowed to grow overnight in LB
Choon Kit, Zhen Fu,Hung:
- Nanodrop to measure concentrations and purities of pFe,pLacI,GFP,RBS32,RBS34,pT7,Terminator. The data show a ratio of A260/A280 varying around 1.85 to 1.95, which mean the purities are quite good.
