Team:Heidelberg/Notebook/Sensing Group/Notebook/2ndweek

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Contents

Monday, 08/11/2008

  • preparation of LuxS harboring cells O/N culture

Tuesday, 08/12/2008

  • Miniprep of correctly sequenced LuxS and Glycerol-stock
  • LuxQ no. 3 checked via digestion with NcoI/BamHI --> negative result
  • PCR for LuxQ, LuxQ-1, LuxQ-2, Tar-1, Tar-2 with Taq Mastermix
  • preparation of O/N culture for LuxQ no. 3

Wednesday, 08/13/2008

Thursday, 08/14/2008

Friday, 08/15/2008

Go to 3rd week