Team:Bologna/Parts

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HOME PROJECT TEAM SOFTWARE MODELING WET LAB LAB-BOOK SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Composites

The table below shows all the composites that were obtained and use to build up our final device. For each part, it ispossible to find:

-the link to the Registry in the second column ;

-part components, in the third column ;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.


N° Igem Code Fragment Plasmid Length (bp) Vitality Fluo
1
 [http://partsregistry.org/Part:BBa_K079015 K079015] LacY transporter protein from E. coli (for the improvement of the registry) [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1AK3 (HC)] 1254
Yes
-
2
 [http://partsregistry.org/Part:BBa_K079017 K079017] Lac symmetric - operator library member GENEART pGA18 20
Yes
-
3
 [http://partsregistry.org/Part:BBa_K079018 K079018] Lac 1 - operator library member GENEART pGA18 21
Yes
-
4
 [http://partsregistry.org/Part:BBa_K079019 K079019] Lac 2 - operator library member GENEART pGA18 21
Yes
-
5
 [http://partsregistry.org/Part:BBa_K079036 K079036] Tet O operator library member GENEART pMA 15
Yes
-
6
 [http://partsregistry.org/Part:BBa_K079037 K079037] TetO-4C - operator library member GENEART pMA 15
Yes
-
7
 [http://partsregistry.org/Part:BBa_K079038 K079038] TetO-wt/4C5G - operator library member GENEART pMA 15
Yes
-
8
 [http://partsregistry.org/Part:BBa_K079039 K079039] LexA 1 - operator library member GENEART pMA 16
Yes
-
9
 [http://partsregistry.org/Part:BBa_K079040 K079040] LexA 2 - opeartor library member GENEART pMA 16
Yes
-
10
 [http://partsregistry.org/Part:BBa_K079041 K079041] Lambda OR1 - operator library member GENEART pMA 17
Yes
-
11
 [http://partsregistry.org/Part:BBa_K079042 K079042] Lambda OR2 - operator library member GENEART pMA 17
Yes
-
12
 [http://partsregistry.org/Part:BBa_K079043 K079043] Lambda OR3 - operator library member GENEART pMA 17
Yes
-
13
 [http://partsregistry.org/Part:BBa_K079045 K079045] Lac operator library GENEART pGA18 78
Yes
-
14
 [http://partsregistry.org/Part:BBa_K079046 K079046] Tet operator library GENEART pMA 61
Yes
-
15
 [http://partsregistry.org/Part:BBa_K079047 K079047] Lambda operator library GENEART pMA 67
Yes
-
16
 [http://partsregistry.org/Part:BBa_K079048 K079048] LexA operator library GENEART pMA 40
Yes
-
17
 [http://partsregistry.org/Part:BBa_K079020 K079020] GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 2165
Yes
>>
18
 [http://partsregistry.org/Part:BBa_K079026 K079026] GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 2116
Yes
>>
19
 [http://partsregistry.org/Part:BBa_K079029 K079029] GFP expression controlled by LacI repressor under the BBa_J23114 promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 2501
Yes
>>
20
 [http://partsregistry.org/Part:BBa_K079049 K079049] GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 981
Yes
-
21
 [http://partsregistry.org/Part:BBa_K079050 K079050] GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 981
Yes
>>
22
 [http://partsregistry.org/Part:BBa_K079023 K079023] Auto-regulated J23118 constitutive promoter and Lac 2 operator [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 1243
Yes
>>
23
 [http://partsregistry.org/Part:BBa_K079024 K079024] LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 1243
Yes
>>
24
 [http://partsregistry.org/Part:BBa_K079030 K079030] GFP reporter protein under the control of the BBa_23105 constitutive promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 957
Yes
>>
25
 [http://partsregistry.org/Part:BBa_K079031 K079031] GFP reporter protein under the control of the BBa_J23118 promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 957
Yes
>>
26
 [http://partsregistry.org/Part:BBa_K079032 K079032] GFP reporter protein under the control of the BBa_J23100 constitutive promoter [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] 957
Yes
>>


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