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Team:TUDelft/Color design
...mes from the registry. Of the eight enzymes we will isolate three are ''E. coli'' enzymes (atoB, idi and ispA), while the other five are ''S. cerevisiae'' To PCR the genes out of the ''E. coli'' genome, we've designed primers using the invitrogen website. These primer

5 KB (764 words) - 17:36, 29 October 2008
Team:Slovenia/Notebook/Methods
... DH5α strain was used. After DNA sequences verification E. coli BL21(DE3)pLysS were transformed with specific constructs for the overex ...ogenization Kit (Novagen) to integrate λDE3 prophage into the E. coli host cell chromosome. λDE3 is a recombinant phage carrying a clo

48 KB (6,976 words) - 04:42, 30 October 2008
Team:University of Ottawa/Project
...s are often expressed in simple microorganisms such as bacteria (e.g. ''E. coli'') or yeast. The two most used yeast species are ''Saccharomyces cerevisiae ...urrently limited when compared to the spectrum of parts developed for ''E. coli'', we thought that this would be a novel yeast-based system to expand upon.

22 KB (3,281 words) - 00:50, 30 October 2008
Team:ETH Zurich/Wetlab/Genome Reduction
...tion we discuss about how to randomly delete chromosomal fragments of ''E. coli'' in order to reduce the physical amount of genomic DNA. ...neArt and is supposed to be integrated into the genome of a wild-type ''E. coli'' strain (MG1655). We are planning to use the lambda red recombination syst

12 KB (1,777 words) - 04:34, 30 October 2008
Team:NTU-Singapore/Wetlab/Materials and Equipment
A colony of E. coli W3110 was grown overnight in LB for 16 hours at 37°C . The overnight cultu ...used in all the experiments were LuxS mutants derived from the wild type E coli strain W3110. By knocking out LuxS gene, the cells were unable to synthesiz

6 KB (869 words) - 05:47, 27 October 2008
Team:Bologna/Biosafety
...ral kinds of bacteria and viruses [including canine hepatitis, Escherichia coli, varicella (chicken pox)], as well as some cell cultures and non-infectious ==Genetically modified ''E. coli''==

29 KB (4,412 words) - 02:19, 30 October 2008
Team:Heidelberg/Project/Sensing
...rjik, 2004). For a schematic presentation of the chemotaxis signaling in E.coli see figure 1. ...ain objective of our project is to engineer a so called killer strain of E.coli which would be able to swim by chemotaxis towards a “prey” strain whic

35 KB (5,074 words) - 22:59, 29 October 2008
User:Greenbear/sandbox
...in E7'' to deal with the pathogenic strain of Ecoli known as ''Escherichia coli O157:H7''. Escherichia coli O157:H7 is an ''enterohemorrhagic strain'' that can cause bloody diarrhea a

2 KB (259 words) - 09:02, 25 May 2008
Team:NTU-Singapore/Project
...NEERING COLICIN E7 PRODUCTION SYSTEM TO KILL ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 (EHEC) VIA THE DETECTION OF EHEC SYMPTOMS </center> ...se of bacteriocins (i.e. colicin E7) for the killing of the Escherichia Coli O157:H7 enterohemorrhagic strain (EHEC), which causes symptoms such as

5 KB (768 words) - 14:55, 28 October 2008
Team:KULeuven/Model/Filter
...s of the ribokey/lock and the difference in transcription rates between E. coli's RNA polymerase II and phage T7's polymerase. ...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” <

15 KB (2,344 words) - 23:12, 29 October 2008
Team:NTU-Singapore/Team/Project
...NEERING COLICIN E7 PRODUCTION SYSTEM TO KILL ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 (EHEC) VIA THE DETECTION OF EHEC SYMPTOMS </center> ...se of bacteriocins (i.e. colicin E7) for the killing of the Escherichia Coli O157:H7 enterohemorrhagic strain (EHEC), which causes symptoms such as

4 KB (577 words) - 15:07, 20 October 2008
Team:Hawaii/Project
:Our toolkit was designed for conjugative gene transfer from ''Escherichia coli'' to ''Synechocystis'' to achieve the controlled production and recovery of ... origin of transfer was confirmed by sequencing and will be tested in ''E. coli'' using the construct with ''oriT'' inserted into the ''lacZ'' BioBrick (J3

7 KB (1,011 words) - 03:46, 30 October 2008
Team:Calgary Wetware/Project
...e signal inputs and subsequently initiating transcription of specific ''E. coli''-targeted bacteriocins (''i.e.'' colicins). The presence of AI-1 induces t ...http://partsregistry.org/Part:BBa_J23039 J23039] was transformed into ''E. coli'' thus resulting in Bad Guy #1 cells (i.e. capable of AHL production). To c

10 KB (1,425 words) - 03:10, 30 October 2008
Team:Heidelberg/Project/Killing I
...l fibres, which bind specifically to mannose transporters of ''Escherichia coli''. These receptors are not able to bind stably to structures on other bacte ...modified phages, a GFP cassette for visualization of the phage and an ''E. coli'' oriT (R), which allows the conjugation of the harboring plasmid, provided

55 KB (8,330 words) - 16:49, 29 October 2008
Team:Slovenia/Results/Engineered flagellin vaccine/Engineered bacterial vaccine
...flagellin. We cloned several constructs, which were transformed into E. coli, strain JW1908-1 (+T7 polymerase and bacterial ghost plasmid (BG)). ...e-stained bacteria expressing chimeric flagellin. Flagellin knockout E. coli strain JW1908-1 was transformed with constructs coding for: T7-CF-AK3,

12 KB (1,625 words) - 04:43, 30 October 2008
Team:TUDelft/Temperature design
...ign phase I: Introducing an RNA thermometer into ''Escherichia coli''= ...e RNA thermometers it has been shown that they can be introduced into ''E. coli'', where they still function as a temperature sensitive switch.

9 KB (1,372 words) - 07:59, 29 October 2008
Team:LCG-UNAM-Mexico/Modeling
Escherichia coli nickel efflux pump ...owe JL and Chivers PT (2006) Nickel homeostasis in Escherichia coli – the rcnR-rcnA efflux pathway and its linkage to NikR function</str

17 KB (2,258 words) - 06:33, 30 October 2008
Team:TUDelft/Temperature design2
.... Note, however, that there will always be an upper bound in that the ''E. coli'' cannot survive temperatures above 40°C, and a lower bound in that the ac ...RNA thermometers for temperature-controlled gene expression in Escherichia coli. ''Nucleic Acids Research'', 1-9, 2008. [http://www.ncbi.nlm.nih.gov/pubmed

20 KB (3,168 words) - 19:33, 29 October 2008
Team:Caltech/Biosafety
*'''Short Term:''' There are no safety concerns beyond that of typical ''E. coli'' lab strains. Considering that 260 mM H2O2 is applie ...K, Schmidt MA, Buer J, and Bruder D. '''Intestinal immunity of Escherichia coli NISSLE 1917: a safe carrier for therapeutic molecules'''. ''FEMS Immunol Me

6 KB (933 words) - 23:57, 29 October 2008
Team:TUDelft/Color analysis
... adding other enzymes. Of the eight enzymes we will isolate three are ''E. coli'' enzymes (atoB, idi and ispA), while the other five are ''S. cerevisiae en ...UDelft/20_August_2008 August 20th]). The next step will be to PCR the ''E. coli'' genes again, with the ideal annealing temperature, using ''Pfx'' polymera

1 KB (234 words) - 13:15, 27 October 2008