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15:17, 21 October 2008 (diff | hist) N Waterloo/13 October 2008 ‎ (New page: 08/10/13 Monday [] patch of transformants [] colony PCR in evening [] prepare stuff to send or resend for sequencing - a few things are now listed on the sequencing f...) (top)
15:15, 21 October 2008 (diff | hist) N Waterloo/12 October 2008 ‎ (New page: 08/10/12 Sunday [] ligation either into a) pJET b) BioBrick vector subcloning [] transformation of ligations [] during next transformation, include: a) ...) (top)
15:10, 21 October 2008 (diff | hist) N Waterloo/11 October 2008 ‎ (New page: 08/10/11 Saturday [] kit prep [] digest [] gel for GEx [] make glycerol stocks [] GEx ) (top)
15:09, 21 October 2008 (diff | hist) N Waterloo/10 October 2008 ‎ (New page: 08/10/10 Friday [x] fill more tubes with LB media [] inoculate stuff to kit prep more DNA of (running low on some of the stuff we need to subclone/assemble) [] inocu...) (top)
15:05, 21 October 2008 (diff | hist) N Waterloo/9 October 2008 ‎ (New page: 08/10/09 Thursday [x] kit prep of cultures inoculated on Wed [x] digest a) above kit preps b) rfp"-2 and -5 (X/P) c) anything from the sequencing results that ...) (top)
13:44, 21 October 2008 (diff | hist) N Waterloo/8 October 2008 ‎ (New page: 08/10/08 Wednesday [x] kit prep POcI [x] digest stuff for cloning (pJET(rfp") - E/S and S/P; pSB2K3(PRecA) - S/P; pSB1A2(POcI) - S/P) [x] gel for GEx: a) digests<...) (top)
13:36, 21 October 2008 (diff | hist) N Waterloo/7 October 2008 ‎ (New page: 08/10/07 Tuesday [x] take picture of Gel from yesterday [x] GEx band from yesterday [] ligation of PrecA into biobrick vector [] transformation [x] Inoculated ...) (top)
13:33, 21 October 2008 (diff | hist) N Waterloo/6 October 2008 ‎ (New page: 08/10/06 Monday [x] prepare and send stuff for sequencing [x] Digest vector for cloning in PrecA [] GEx vector ) (top)
13:29, 21 October 2008 (diff | hist) N Waterloo/5 October 2008 ‎ (New page: 08/10/05 Sunday [x] kit prep cultures from Saturday ) (top)
13:28, 21 October 2008 (diff | hist) N Waterloo/4 October 2008 ‎ (New page: 08/10/04 Saturday [x] check gel of colony PCR [x] Inoculate positive colonies ) (top)
13:27, 21 October 2008 (diff | hist) N Waterloo/3 October 2008 ‎ (New page: 08/10/03 Friday [x] Patch transformants [x] set up colony PCR [] Make gel for tomorrow) (top)
13:23, 21 October 2008 (diff | hist) N Waterloo/2 October 2008 ‎ (New page: 08/10/02 Thursday [] Patch again - Transformation didn't work [x] pour Km and Ap plates [x] digest and run gel [x] Gel extraction of digest [x] ligation<br...) (top)
19:47, 20 October 2008 (diff | hist) N Waterloo/18 September 2008 ‎ (New page: 08/09/18 Thursday [x] continue with crossover PCR - run with KOD at various DMSO% - RFP' in the G box should be used as positive control using VR and Pre+RBS primers a...) (top)
19:44, 20 October 2008 (diff | hist) N Waterloo/17 September 2008 ‎ (New page: 080917 Wed [x] rerun diagnostic digest and gel on potential BioBrick subclones using Buffer O, since it's just a NotI single digest [x] make lots of media! (fill 5 mL tube...) (top)
19:41, 20 October 2008 (diff | hist) N Waterloo/16 September 2008 ‎ (New page: 080916 Tue [x] run gel of PCR products [x] based on above results, set up PCR of best conditions using Charles lab KOD Hot Start [] make lots of liquid media! (fill...) (top)
19:39, 20 October 2008 (diff | hist) Waterloo/12 September 2008 ‎ (top)
19:38, 20 October 2008 (diff | hist) N Waterloo/15 September 2008 ‎ (New page: 080915 Mon [x] rerun diagnostic digest and gel on potential BioBrick subclones - seems to have contamination again [x] run PCR of crossover stuff using Taq master mix ...) (top)
19:36, 20 October 2008 (diff | hist) N Waterloo/12 September 2008 ‎ (New page: 08/09/12 Friday [] based on gel results, get stuff ready to send for sequencing - gel results looked terrible therefore no sequencing for Friday [] send promising Thurs...)
19:33, 20 October 2008 (diff | hist) N Waterloo/11 September 2008 ‎ (New page: 080911 Thu [x] kit prep inoculated cultures from Wednesday, Sept. 10 [x] diagnostic digest of above [x] diagnostic gel of above ) (top)
19:32, 20 October 2008 (diff | hist) N Waterloo/10 September 2008 ‎ (New page: 080910 Wed [x] order crossover PCR primers [x] make CaCl2 competent E. coli of strain TG1 which contains lacI for Plac testing - Box E, 10 500uL aliquots [x] anal...) (top)
19:24, 20 October 2008 (diff | hist) Waterloo/9 September 2008 ‎ (top)
19:21, 20 October 2008 (diff | hist) N Waterloo/8 September 2008 ‎ (New page: 080908 Mon [x] check Sunday's transformation results Putative good constructs yet to be sequenced: [x] inoculate culture from transformants of parts for sequencing (an...) (top)
19:21, 20 October 2008 (diff | hist) Waterloo/9 September 2008 ‎
19:18, 20 October 2008 (diff | hist) N Waterloo/9 September 2008 ‎ (New page: 080908 Mon [x] check Sunday's transformation results Putative good constructs yet to be sequenced: [x] inoculate culture from transformants of parts for sequencing (an...)
19:10, 20 October 2008 (diff | hist) N Waterloo/7 September 2008 ‎ (New page: 080907 Sun [x] redo transformation: a) parts to sequence b) synthesized parts (Ap) [x] inoculate cultures from: a) Saturday's transformants (only gene6 and Pm...) (top)
19:06, 20 October 2008 (diff | hist) N Waterloo/6 September 2008 ‎ (New page: 080906 Sat [x] compile list of things to sequence this week [x] run check gel of GEx products - everything looked good (had ~correctly sized bands for each GEx) [x...) (top)
18:55, 20 October 2008 (diff | hist) N Waterloo/5 September 2008 ‎ (New page: 080905 Fri [x] analyze Thursday's gel; select which parts to move forward with [x] redo diag digest and gel of gene6 vs. putative gene6' - gene6' looks too much larg...) (top)
18:49, 20 October 2008 (diff | hist) N Waterloo/4 September 2008 ‎ (New page: 080904 Thu [x] redo diag of things that didn't work [x] fill tips, tubes, etc. [x] annealed fwd and rev oligos for pRecA ) (top)
18:48, 20 October 2008 (diff | hist) N Waterloo/3 September 2008 ‎ (New page: 080903 Wed [x] run NotI diagnostic gel: - pJET(gene6) vs. pSB1A2(RBS-gene6?) --> [if looks okay, send for sequencing] - cI'? (both C0051'? and C0052'?) (in good box) ...) (top)
18:46, 20 October 2008 (diff | hist) Waterloo/28 August 2008 ‎ (top)
18:44, 20 October 2008 (diff | hist) N Waterloo/28 August 2008 ‎ (New page: 08/08/28 Thurs - Last day for coops [] miniprep of c0051'', c0052'' [] diag digest [] diag gel )
18:42, 20 October 2008 (diff | hist) N Waterloo/27 August 2008 ‎ (New page: 08/08/27 Wed - Bioseminar presentation [] frozen stock of transformants [] p/s/i of transformation (c0051'', c0052'') ) (top)
18:41, 20 October 2008 (diff | hist) N Waterloo/26 August 2008 ‎ (New page: 08/08/26 Tues [] competent cells [] gel of full digestion (c0051', c0052', TT, TT, RBS, RBS) [] gel extraction [] ligation [] transformation [] inoculate...) (top)
18:34, 20 October 2008 (diff | hist) N Waterloo/25 August 2008 ‎ (New page: 08/08/25 Mon [x] frozen stock of pJET + gene 6 (27 and 29) [x] diagnostic digest of gene6' results - biobrick double digest (do not have the appropriate enzyme in stock to ...) (top)
18:30, 20 October 2008 (diff | hist) N Waterloo/24 August 2008 ‎ (New page: 08/08/24 Sun [x] reinoculate pJET + gene 6 [] inoculate 2 tubes each of a couple RBS-gene6 samples that looked good based on Friday's colony PCR results - Monday: make...) (top)
18:23, 20 October 2008 (diff | hist) N Waterloo/22 August 2008 ‎ (New page: 08/08/22 Fri [x] miniprep of TT, RBS, 8 samples [] prepare list for transformations ) (top)
18:21, 20 October 2008 (diff | hist) N Waterloo/21 August 2008 ‎ (New page: 08/08/21 Thurs [x] gel of full digestion digestion - not left long enough, will repeat - TT did not work, will reinoculate [] gel extraction [] ligation []...) (top)
18:07, 20 October 2008 (diff | hist) N Waterloo/20 August 2008 ‎ (New page: 08/08/20 Wed [x] miniprep of inoculatants [x] diagnostic digestion [x] diag gel of inoculants and PCR from Tues [] diagnostic gel of sketchy DNA [] gel and ...) (top)
17:59, 20 October 2008 (diff | hist) N Waterloo/19 August 2008 ‎ (New page: 08/08/19 Tues [x] inoculate good colonies from colony PCR [x] glycerol stock of iGEM stabs [x] repeat PCR phb testing - Try the ones that didn't work again, thi...) (top)
17:56, 20 October 2008 (diff | hist) N Waterloo/18 August 2008 ‎ (New page: 08/08/18 Mon [x] check gel of PHB PCR products [x] GEx of PHB PCR products (if PCR successful based on check gel) [x] colony PCR of c0051', c0052', gene 6' [x] di...) (top)
17:52, 20 October 2008 (diff | hist) N Waterloo/17 August 2008 ‎ (New page: 08/08/17 Sun [x] PCR of PHB A, B, C genes using genomic DNA and Phusion polymerase - Try it with the 1:100 dilution of Rm1021 CB (cosmid bank) DNA, left in the "good const...) (top)
17:40, 20 October 2008 (diff | hist) N Waterloo/15 August 2008 ‎ (New page: 08/08/15 Fri [x] diag gel of PCR from yesterday [x] PCR (colony PCR testing with RBS) [x] diag gel of PCR ) (top)
17:37, 20 October 2008 (diff | hist) N Waterloo/14 August 2008 ‎ (New page: 8/08/14 Thurs [x] send gene 6 for sequencing [] p/s/i of c0052', c0051', T7 gene 6 + RBS [x] miniprep of inoculatants from wed [x] Gel of overnight PCR of phbA ...) (top)
17:28, 20 October 2008 (diff | hist) N Waterloo/13 August 2008 ‎ (New page: 08/08/13 Wed [x] gel of full digestion (c0052', c0051', T7 gene6-27) [x] gel extraction [x] check gel of extraction [x] ligation of parts from extraction and c0052...) (top)
17:16, 20 October 2008 (diff | hist) N Waterloo/12 August 2008 ‎ (New page: 08/08/12 Tues [x] kit prep T7 gene 6 and gene 5 - High Priority [x] diagnostic digest of kit prep of gene 6&5 - Double (e/s) and Single (e) [] run gel of diagnost...) (top)
17:12, 20 October 2008 (diff | hist) N Waterloo/11 August 2008 ‎ (New page: 08/08/11 Mon [x] transformation of T7 gene 5 (4) in pJET [x] Inoculation of gene 5&6 colonies from pJET transformants [x] inoculate c0051' and c0052' [x] inoculate ...) (top)
17:09, 20 October 2008 (diff | hist) N Waterloo/10 August 2008 ‎ (New page: 10/08/08 Sun [] Inoculation of gene 5&6 colonies from pJET transformants - no one was able to come in for this) (top)
17:06, 20 October 2008 (diff | hist) N Waterloo/9 August 2008 ‎ (New page: 09/08/08 Sat [x] gel of PCR T7 gene 5 from friday [x] gel extraction of PCR [x] pJET ligation [x] transformation ) (top)
20:28, 17 October 2008 (diff | hist) N Waterloo/7 August 2008 ‎ (New page: 08/08/07 Thurs [] p/s/i of c0051' and c0052' - will wait until colonies are bigger [x] repeat of test PCR T7 gene 5 [x] diagnostic gel of PCR [] if colonies are b...) (top)
20:26, 17 October 2008 (diff | hist) N Waterloo/8 August 2008 ‎ (New page: 08/08/08 Fri [x] colony PCR of T7 gene 6, c0051', c0052' [x] repeat of PCR t7 gene 5 [x] diag gel of colony pcr and t7 gene 5 PCR ) (top)

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