"
User contributions
From 2008.igem.org
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 17:57, 22 October 2008 (diff | hist) Team:Mississippi State/Project
- 18:19, 16 October 2008 (diff | hist) Team:Mississippi State/9 September 2008 (top)
- 18:17, 16 October 2008 (diff | hist) N Team:Mississippi State/9 September 2008 (New page: *pick colonies (12) *extract plasmid)
- 18:16, 16 October 2008 (diff | hist) N Team:Mississippi State/8 September 2008 (New page: *prepare ligation mix of pgem and atail gene *spread on plates after transformation) (top)
- 18:16, 16 October 2008 (diff | hist) N Team:Mississippi State/3 September 2008 (New page: *run gel on pcr product *add a tail using pfx50) (top)
- 18:15, 16 October 2008 (diff | hist) N Team:Mississippi State/2 September 2008 (New page: *prepare pcr mix of lip template using taq) (top)
- 18:14, 16 October 2008 (diff | hist) Team:Mississippi State/Papers (→Notebook)
- 18:14, 16 October 2008 (diff | hist) N Team:Mississippi State/28 August 2008 (New page: *check plates *extract plasmid *no results) (top)
- 18:13, 16 October 2008 (diff | hist) N Team:Mississippi State/27 August 2008 (New page: *inoculate cells *prepare ligation mix for pGEM and pcr product *make competent cells *transform pGEM in to e coli) (top)
- 18:11, 16 October 2008 (diff | hist) N Team:Mississippi State/26 August 2008 (New page: *make Amp plates) (top)
- 18:11, 16 October 2008 (diff | hist) N Team:Mississippi State/25 August 2008 (New page: *run gel to see how much gene product is left *freeze dry 8/19 pcr product *start xlIblue cells overnight culture) (top)
- 18:09, 16 October 2008 (diff | hist) N Team:Mississippi State/22 August 2008 (New page: *extract plasmid *run gel *digest plasmid --> no insert present) (top)
- 18:08, 16 October 2008 (diff | hist) N Team:Mississippi State/20 August 2008 (New page: *inoculate cells *make competent cells *do transformation with pGEM) (top)
- 18:07, 16 October 2008 (diff | hist) N Team:Mississippi State/19 August 2008 (New page: * prepare and run gel *cut out and purify pcr product *prepare pGEM ligation mix using pcr product) (top)
- 18:06, 16 October 2008 (diff | hist) N Team:Mississippi State/18 August 2008 (New page: *do pcr w/ 2nd primers and taq *run 2nd pcr with different template (RP1)) (top)
- 18:05, 16 October 2008 (diff | hist) N Team:Mississippi State/15 August 2008 (New page: *prepare and run gel on plasmid extraction *digest plasmid to check for gene *there was no insert, and mistake was made in not adding IPTG and PGAL to the plates. *Dr Ma: do pcr w/ lip usi...) (top)
- 18:03, 16 October 2008 (diff | hist) N Team:Mississippi State/14 August 2008 (New page: *pick 10 colonies and grow 10hrs *extract plasmid) (top)
- 18:02, 16 October 2008 (diff | hist) N Team:Mississippi State/13 August 2008 (New page: *inoculate xlIblue for 2hr *prepare and run gel *prepare ligation mix *make competent cells *perform transformation) (top)
- 18:01, 16 October 2008 (diff | hist) N Team:Mississippi State/12 August 2008 (New page: *redigest Lip and pPIC6 with EcoRI and NotI *Prepare ligation mix *prepare PCR mix to make more of the gene stock *prepare and run 1% agarose gel *purify PCR product *digest pcr w/ notI an...) (top)
- 17:58, 16 October 2008 (diff | hist) N Team:Mississippi State/11 August 2008 (New page: =August 11, 2008= *pick 10 colonies and grow in 2ml LB, 100ug/ml Blast 10hrs. *extract plasmid using Qiagen Kit *prepare and run 10% agarose gel) (top)
- 18:19, 13 October 2008 (diff | hist) Team:Mississippi State (top)
- 18:18, 13 October 2008 (diff | hist) N File:Msuigem08.JPG (top)
- 18:10, 10 October 2008 (diff | hist) Team:Mississippi State/Project
- 15:58, 10 October 2008 (diff | hist) Team:Mississippi State/Tshirts (top)
- 15:57, 10 October 2008 (diff | hist) Team:Mississippi State/Tshirts
- 15:57, 10 October 2008 (diff | hist) Team:Mississippi State/Tshirts
- 15:55, 10 October 2008 (diff | hist) Team:Mississippi State/Tshirts
- 15:54, 10 October 2008 (diff | hist) N Team:Mississippi State/Tshirts (New page: [Image:MStateblob.JPG|center|300 px] [Image:Igemtshirts.JPG|center])
- 15:52, 10 October 2008 (diff | hist) N File:Igemtshirts.JPG (top)
- 15:52, 10 October 2008 (diff | hist) N File:MStateblob.JPG (top)
- 15:51, 10 October 2008 (diff | hist) Team:Mississippi State/Team
- 15:24, 3 October 2008 (diff | hist) Team:Mississippi State/Team
- 15:14, 3 October 2008 (diff | hist) Team:Mississippi State/Project
- 15:13, 3 October 2008 (diff | hist) Team:Mississippi State/Project
- 15:12, 3 October 2008 (diff | hist) Team:Mississippi State/Project
- 15:12, 3 October 2008 (diff | hist) Team:Mississippi State/Project
- 15:10, 3 October 2008 (diff | hist) Team:Mississippi State/Papers
- 15:10, 3 October 2008 (diff | hist) Team:Mississippi State/Project
- 15:09, 3 October 2008 (diff | hist) Team:Mississippi State/Team
- 15:08, 3 October 2008 (diff | hist) Team:Mississippi State
- 15:07, 3 October 2008 (diff | hist) Team:Mississippi State
- 15:07, 3 October 2008 (diff | hist) Team:Mississippi State
- 19:25, 11 August 2008 (diff | hist) N Team:Mississippi State/7 August 2008 (New page: # take plates out and save # re-digest pPIC6 #2 # prepare and run 1% agarose gel # make now low salt plates # purify gel cut-out # make ligation mix using T4, Lip, and pPIC6 # perform tran...) (top)
- 19:22, 11 August 2008 (diff | hist) N Team:Mississippi State/6 August 2008 (New page: # prepare 10 tubes for growing colonies # incubate @37C 10hr # extract plasmid using qiagen kit # prepare and run 1% agarose gel) (top)
- 19:21, 11 August 2008 (diff | hist) N Team:Mississippi State/5 August 2008 (New page: # prepare 20% glycerol yeast cell stock # check plates for colonies # make low salt LB and plates # inoculate cells # prepare competent cells # perform transformation using remaining ligat...) (top)
- 19:19, 11 August 2008 (diff | hist) N Team:Mississippi State/4 August 2008 (New page: # Repeat digestion protocol using NotI, EcoRI, LiP, and pPIC6 # prepare and run 1% agarose gel, cut out products # perform gel purification using QIAgen kit # prepare and run 1% agarose ge...) (top)
- 19:16, 11 August 2008 (diff | hist) N Team:Mississippi State/1 August 2008 (New page: # prepare and run 1% agarose gel with plasmid extraction products. # run control to see if EcoRI and NotI are functional # Prepare and run 1% agarose gel- both enzymes cut normally) (top)
- 19:14, 11 August 2008 (diff | hist) Team:Mississippi State/Papers (→Notebook)
- 19:13, 11 August 2008 (diff | hist) N Team:Mississippi State/31 July 2008 (New page: # Make 25ug/ul blast stock # make low salt lb w/ 50ug/ml blast # Incubate 10 colonies picked from plates 10hrs # extract plasmid using P1,P2,P3) (top)
- 19:11, 11 August 2008 (diff | hist) N Team:Mississippi State/30 July 2008 (New page: # inoculate cells # extract plasmid(additional pPIC6 stock) # prepare competent cells # prepare and run 1% agarose gel # perform transformation with Lip/pPIC6 ligation mix # spread on plat...) (top)
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
