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User contributions
From 2008.igem.org
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- 03:33, 27 October 2008 (diff | hist) m Team:Virginia/Sandbox
- 03:16, 27 October 2008 (diff | hist) m Team:Virginia/Sandbox
- 01:38, 27 October 2008 (diff | hist) m Team:Virginia/Sandbox
- 01:13, 27 October 2008 (diff | hist) N Virginia/26 October 2008 (New page: *Cut A77, A79, A80 (E,S) to assemble with terminator **Rationale is that maybe having the promoter in front of the coding region with no terminator is killing the cells *Gelled A82(3) (E,P...) (top)
- 01:11, 27 October 2008 (diff | hist) N Virginia/25 October 2008 (New page: ==Overnight Results== *No colonies ==Accomplished== *Retransformed (with more DNA, 4ul) and plated the same assemblies again Back to Notebook) (top)
- 01:10, 27 October 2008 (diff | hist) m Virginia/24 October 2008 (top)
- 01:09, 27 October 2008 (diff | hist) N Virginia/24 October 2008 (New page: *Ligated A81(2), A83(2) **All using gel-extracted DNA *Ligated A84 **R0040 from gel-extracted DNA, A80 from molten gel Back to Notebook)
- 00:51, 27 October 2008 (diff | hist) N Virginia/23 October 2008 (New page: *Miniprepped A82(3) **First measurement vector! *Cut A82(3) (E,P) **To check length Back to Notebook) (top)
- 00:37, 27 October 2008 (diff | hist) N Virginia/21 October 2008 (New page: *Cut A81-1,2 (E,S) and A77(4)-1,2 (X,P) *Concentrations of DNA run through gel extraction kit is very low **That DNA in a gel looks OK tho Back to Notebook) (top)
- 04:24, 21 October 2008 (diff | hist) N Virginia/20 October 2008 (New page: *Miniprepped A81-1,2 and A77(4)-1,2 *Purified A66(3) (E,S), A79(2) (X,P), E0240 (E,X) and R0040 (S,P) with a gel purification kit **Ran through gel, then kit *Ligated A82(3) and A83 (R0040...) (top)
- 04:16, 21 October 2008 (diff | hist) m Virginia/19 October 2008 (top)
- 04:12, 21 October 2008 (diff | hist) m Virginia/18 October 2008 (top)
- 04:12, 21 October 2008 (diff | hist) N Virginia/19 October 2008 (New page: ==Overnight Results== *A77(4) and A81 had colonies *A82 had no colonies *Butanolysis run: Conc of P3HB - 300 ul of 17.2ug of P3HB ==Accomplished== *Replated A82 from saved cells *Ligated...)
- 04:09, 21 October 2008 (diff | hist) N Virginia/18 October 2008 (New page: *Liagated and plated A81 (R0040 + A80) *Cut A79(2)-1,2 (X,P) *Ligated A77(4) Back to Notebook)
- 04:07, 21 October 2008 (diff | hist) m Virginia/17 October 2008 (top)
- 04:05, 21 October 2008 (diff | hist) N Virginia/17 October 2008 (New page: ==Overnight Results== *A77(3), A78(3) and A80(3) didn't grow ==Accomplished== *Discussed Real Time PCR with Misty (grad student) *Added SOC to saved cells of A77(3), A78(3) and A80(3), r...)
- 04:03, 21 October 2008 (diff | hist) N Virginia/15 October 2008 (New page: *Nanodropped DNA **A66(3), A79(2)-1,2 all look good *Religated A77(3), A78(3) and A80(3) *Cut A66(3) (E,S) *Gel of E0240(E,X) and A66(3)(E,S) looks good **Cut out parts [[Team:Virginia/N...) (top)
- 04:01, 21 October 2008 (diff | hist) N Virginia/14 October 2008 (New page: ==Overnight Results== *Broths look good Back to Notebook) (top)
- 00:56, 14 October 2008 (diff | hist) N Virginia/13 October 2008 (New page: ==Overnight Results== *Only 1 plate had colonies (A79(2)) *A66(3) had a single red colony ==Accomplished== *Purified more I715039(E,S) with low melt *Broth of A66(3) red colony and also o...) (top)
- 15:29, 12 October 2008 (diff | hist) N Virginia/11 October 2008 (New page: *Re-did A77-80 *Ligated A81 (R0040 + A80) *Ligated A66(3) (I715039 + B0015) *Transformed and plated all of the above Back to Notebook) (top)
- 04:32, 8 October 2008 (diff | hist) N Virginia/7 October 2008 (New page: *Miniprepped A76-80 **Good concentrations Back to Notebook) (top)
- 23:42, 6 October 2008 (diff | hist) N Virginia/6 October 2008 (New page: ==Overnight Results== *All plates (A76-80) had colonies **A76 had a lot, the others had a decent number ==Accomplished== *Made small overnight broth of A76-80 **Made 2 from A76, one from ...) (top)
- 00:15, 6 October 2008 (diff | hist) N Virginia/5 October 2008 (New page: *Transformed and plated A76 (RFP + GFP) *Ligated A77-80 **A77 = B0032 + OFP **A78 = B0034 + OFP **A79 = B0032 + BFP **A80 = B0034 + BFP *Transformed and plated A77-80 [[Team:Virginia/Not...) (top)
- 21:44, 3 October 2008 (diff | hist) N Virginia/3 October 2008 (New page: *Cut I715039 (S,P) + E0240 (X,P) **For baseline plasmid *Ligated A76 (I715039 + E0240) Back to Notebook) (top)
- 20:58, 3 October 2008 (diff | hist) m Virginia/1 October 2008 (top)
- 20:56, 3 October 2008 (diff | hist) N Virginia/1 October 2008 (New page: ==Overnight Results== *Positive and negative control plates were right *Colonies on plates for A73, A75 **Made small overnight broth for those two *Gel of cut OFP and BFP looks right **Two...)
- 20:54, 3 October 2008 (diff | hist) N Virginia/28 September 2008 (New page: *Ran gel of A73-75 (E,P) **Looks right, A75 had no band though (Probably only cut once) Back to Notebook) (top)
- 20:52, 3 October 2008 (diff | hist) N Virginia/26 September 2008 (New page: *Measured DNA concentrations A73-75 **pretty low, 20, 43, 49 ul/ml Back to Notebook) (top)
- 20:52, 3 October 2008 (diff | hist) N Virginia/16 September 2008 (New page: *Cut A70-72 (X,P) *Added sterile H2O (50ul) to latest gene received from GeneART Back to Notebook) (top)
- 03:00, 1 October 2008 (diff | hist) N Virginia/30 September 2008 (New page: *Cut BFP and OFP with (X,P) *Transformed and plated A73-A75 and positive (pUC19) and negative controls *Made broth of same also Back to Notebook) (top)
- 19:09, 24 September 2008 (diff | hist) m Team:Virginia/Sandbox
- 03:58, 24 September 2008 (diff | hist) m Team:Virginia/Sandbox (Undo revision 47821 by DRTarjan (Talk))
- 03:57, 24 September 2008 (diff | hist) m Team:Virginia/Sandbox
- 03:45, 24 September 2008 (diff | hist) m Team:Virginia/Sandbox
- 03:38, 24 September 2008 (diff | hist) m Team:Virginia/Sandbox
- 03:35, 24 September 2008 (diff | hist) N File:Team2.png (top)
- 03:35, 24 September 2008 (diff | hist) N File:Rsbp.png (top)
- 03:35, 24 September 2008 (diff | hist) N File:Parts2.png (top)
- 03:35, 24 September 2008 (diff | hist) N File:Overview.png (top)
- 03:34, 24 September 2008 (diff | hist) N File:Notebook2.png (top)
- 03:34, 24 September 2008 (diff | hist) N File:Modeling2.png (top)
- 03:34, 24 September 2008 (diff | hist) N File:Genetic attenuator.png (top)
- 03:34, 24 September 2008 (diff | hist) N File:Bioplastic.png (top)
- 03:21, 24 September 2008 (diff | hist) m Team:Virginia/Sandbox (fixed error of wiki that left #content in open <p>)
- 02:52, 24 September 2008 (diff | hist) File:Pixel-logo.jpg (uploaded a new version of "Image:Pixel-logo.jpg") (top)
- 02:51, 24 September 2008 (diff | hist) File:Pixel-logo.jpg (uploaded a new version of "Image:Pixel-logo.jpg": Reverted to version as of 02:46, 24 September 2008)
- 02:51, 24 September 2008 (diff | hist) File:Pixel-logo.jpg (uploaded a new version of "Image:Pixel-logo.jpg")
- 02:48, 24 September 2008 (diff | hist) File:Rotunda.JPG (uploaded a new version of "Image:Rotunda.JPG") (top)
- 02:46, 24 September 2008 (diff | hist) File:Pixel-logo.jpg (uploaded a new version of "Image:Pixel-logo.jpg")
- 02:39, 24 September 2008 (diff | hist) m Team:Virginia/Sandbox
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