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User contributions
From 2008.igem.org
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- 21:47, 29 October 2008 (diff | hist) Team:Princeton/ProjectDetails
- 21:44, 29 October 2008 (diff | hist) Template:PrincetonHeader (Undo revision 96017 by Lphalen (Talk))
- 21:43, 29 October 2008 (diff | hist) Template:PrincetonHeader (Undo revision 96055 by Lphalen (Talk))
- 21:42, 29 October 2008 (diff | hist) Template:PrincetonHeader
- 21:39, 29 October 2008 (diff | hist) Template:PrincetonHeader
- 21:18, 29 October 2008 (diff | hist) Team:Princeton/ProjectDetails
- 10:39, 29 October 2008 (diff | hist) Re-transform with selected plasmid (→Transformation Protocol)
- 02:53, 29 October 2008 (diff | hist) Restriction Map/ Restriction Digest (→Restriction Map Design)
- 02:53, 29 October 2008 (diff | hist) Restriction Map/ Restriction Digest (→Restriction Map Design)
- 02:52, 29 October 2008 (diff | hist) Restriction Map/ Restriction Digest (→Restriction Digest Protocol)
- 02:51, 29 October 2008 (diff | hist) Restriction Map/ Restriction Digest (→Restriction Digest Protocol)
- 02:49, 29 October 2008 (diff | hist) Restriction Map/ Restriction Digest (→Restriction Digest Protocol)
- 02:25, 29 October 2008 (diff | hist) Restriction Map/ Restriction Digest (→Restriction Digest Protocol)
- 02:15, 29 October 2008 (diff | hist) Restriction Map/ Restriction Digest (→Restriction Map Design)
- 02:13, 29 October 2008 (diff | hist) Restriction Map/ Restriction Digest (→Restriction Map Design)
- 02:02, 29 October 2008 (diff | hist) N Transfection (nonviral) (New page: ==Transfection Protocol== Take media off of cells Rinse with PBS Put 1mL trypsin on cells. Trypsin will interfere with “sticky” extracellular proteins. When cells start to detach f...)
- 01:58, 29 October 2008 (diff | hist) N Infection (New page: ==Infection Protocol== Count cells. Add 1x105 cells to each well. Add 0.8 ul polybrene for each ml of media in the well. Slide to mix. Add virus – one tube (~15ul) to each well. (B...)
- 01:55, 29 October 2008 (diff | hist) N Lentivirus Harvesting (New page: ==Virus Harvesting Protocol== Set centrifuge to 4C Filter media with cells from virus production plate using 0.2um or 0.22um filter to sterilize. The virus will go through the filter; ...)
- 01:52, 29 October 2008 (diff | hist) Lentivirus Production (→Virus Production Protocol)
- 01:49, 29 October 2008 (diff | hist) N Lentivirus Production (New page: ==Virus Production Protocol== Aspirate 3 large dishes of HEK-293 cells. Add 12.5 mL of normal cell media to each dish. Return the 3 dishes to the incubator. In a 15mL tube, add DMEM,...)
- 01:40, 29 October 2008 (diff | hist) Extract DNA (Maxiprep or Midiprep) (→Midiprep Protocol (QIAGEN kit))
- 01:35, 29 October 2008 (diff | hist) Extract DNA (Miniprep) (→Miniprep Protocol (QIAGEN Miniprep kit))
- 01:28, 29 October 2008 (diff | hist) Extract DNA (Miniprep) (→Miniprep Protocol (QIAGEN Miniprep kit))
- 01:27, 29 October 2008 (diff | hist) Transformation and Plating (→Transformation Protocol)
- 01:21, 29 October 2008 (diff | hist) PCR Purification (→PCR Purification)
- 01:18, 29 October 2008 (diff | hist) Ligate DNA (→Ligation Protocol)
- 01:15, 29 October 2008 (diff | hist) PCR Purification (→PCR Purification)
- 01:14, 29 October 2008 (diff | hist) PCR Purification (→PCR Purification)
- 01:09, 29 October 2008 (diff | hist) CIP Treatment (→CIP Treatment Protocol)
- 01:07, 29 October 2008 (diff | hist) Digestion (→Digestion Protocol)
- 01:02, 29 October 2008 (diff | hist) Run Gel/ Gel extraction (→Run Gel Protocol)
- 01:00, 29 October 2008 (diff | hist) PCR Amplification (→PCR Protocol)
- 00:39, 29 October 2008 (diff | hist) PCR Amplification (→PCR Protocol)
- 00:38, 29 October 2008 (diff | hist) PCR Amplification (→PCR Protocol)
- 00:32, 29 October 2008 (diff | hist) Sequence (→Sequencing Protocol)
- 00:31, 29 October 2008 (diff | hist) Sequence (→Sequencing Protocol)
- 00:30, 29 October 2008 (diff | hist) Sequence (→Sequencing Protocol)
- 00:30, 29 October 2008 (diff | hist) Sequence (→Sequencing Protocol)
- 00:27, 29 October 2008 (diff | hist) Sequence (→Sequencing Protocol)
- 00:12, 29 October 2008 (diff | hist) Ligate DNA (→Ligation Protocol)
- 00:09, 29 October 2008 (diff | hist) Ligate DNA (→Ligation Protocol)
- 00:04, 29 October 2008 (diff | hist) Restriction Map/ Restriction Digest (→Restriction Map Protocol)
- 00:01, 29 October 2008 (diff | hist) Ligate DNA (→Ligation Protocol)
- 23:50, 28 October 2008 (diff | hist) CIP Treatment
- 23:45, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 18:31, 28 October 2008 (diff | hist) Extract DNA (Maxiprep or Midiprep)
- 18:28, 28 October 2008 (diff | hist) Run Gel/ Gel extraction
- 18:28, 28 October 2008 (diff | hist) Run Gel/ Gel extraction (→Protocol for Running a Gel)
- 18:27, 28 October 2008 (diff | hist) PCR Amplification
- 18:15, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
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