"

 

From 2008.igem.org

>Home | Notebook

8 September 2008 <|> 10 September 2008

Contents 1 Laboratory work 1.1 Team:Input 1.2 Team:Communication 1.3 Team:Output

Laboratory work

Team:Input

no work

Team:Communication

(6/9)--->PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030]

--->Gel Extract

Sample DNA 60 Loading Dye 12 TOTAL 72μL CinI

--->Zymo Clean

• CinI

[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]

[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]

[http://partsregistry.org/Part:BBa_S03154 BBa_S03154]

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]

--->Gel Check

Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6μL From left; CinI -> ? [http://partsregistry.org/Part:BBa_C0161 BBa_C0161] -> OK [http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> OK [http://partsregistry.org/Part:BBa_S03154 BBa_S03154] -> OK (low) Marker [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] -> OK (low)

Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6μL [http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK

1. LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
2. LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
3. LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
4. Background([http://partsregistry.org/Part:BBa_R0010 R0010])
5. Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])

--->Ligation

Sample No.	(1)	(2)	(3)	(4)	(5)
insert-C0161	3	-	-	-	-
insert-C0261	-	2	-	-	2
insert-S03154	-	-	6	-	-
Vector-R0010	-	5	5	5	-
vector-J04500	3	-	-	-	-
ligase	1	1	1	1	1
Buffer	2	2	4	2	2
dH2O	1	-	4	2	5
TOTAL	10	10	20	10	10

---> Transformation

---> Colony Count( 10/9)

Team:Output

no work

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Permanent link
• Privacy policy
• Disclaimers