Team:Paris/Modeling/f0

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Method & Algorithm : ƒ0


The determination of ƒ0 is really simple :

Specific Plasmid Characterisation for ƒ0

According to the characterization plasmid (see right) and to our modeling, in the exponential phase of growth, at the steady state, we have

EqF0.jpg

↓ Table of Values ↑


param signification unit value comments
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration
↓ gives ↓ 		nM.au-1 (1/79.429)
[GFP] GFP concentration at steady-state nM
γGFP dilution-degradation rate
of GFP(mut3b)
↓ gives ↓ 		min-1 0.0198 Only dilution :
Time Cell Division : 35 min.
ƒ0 activity of
J23101 with RBS E0032 		nM.min-1


That will directly give us ƒ0



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