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From 2008.igem.org

FIFO Construction Description Validation Date pFliL(RBS WT)-ECFP tripart (LVA+) Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFLiL is ECFP. October 24 pFliL(+ RBS WT)-GFP October 24 pFlgA(+ RBS WT) - YFP Tripart (LVA+) August 22 pFlgA(+ RBS WT) - YFP Tripart (LVA-) August 22 pFlgA(+ RBS WT)-YFP tripart (AAV+) October 24 pFlgA(+ RBS WT)-GFP October 24 pFlhB(+ RBS WT)-mRFP tripart (+LVA) October 24 pFlhB(+ RBS WT)-GFP October 26 OSCILLATION pFliL(+ RBS WT)-EnvZ* October 26 pFliL(+ RBS WT)-EnvZ*-GFP generator October 29 pFlhB(+ RBS WT)-EnvZ* October 24 pFlhB(+ RBS WT)-EnvZ*-GFP generator October 29 araC/pBad-EnvZ* October 27 SYNCHRONISATION pLas - gfp Tripart August 1 pLas - ECFP August 1 strongest promoter-rbs-lasR-dble ter August 20 Strong promoter-rbs-lasR-dble ter August 20 Strongest RBS (1)- LasR activator (+LVA) August 13 rbs-lasR + dbl ter August 19 lasR activator + LVA - Double Terminator August 1 RBS lasI - GFP tripart August 13 RBS-lasI - ECFP August 13 NEW GENE AND PROMOTER BIOBRICKS pFliA October 04 pFliL (+RBS WT) October 10 pFlgA (+RBS WT) October 10 pFlgB (+RBS WT) October 10 pFlhB (+RBS WT) October 10 EnvZ* September 04 FliA September 04 FlhDC August 13

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