Team:NTU-Singapore/Notebook/30 June 2008

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  • Chin Chong
    • Stock check of items purchased so far and shifted them into new lab with Darius's help
    • Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed the purchase and sequence should arrive in 2-4 weeks
    • Purchased items from SBS general store
    • Did inoculation of colonies from Top cells containing:
  1. LacI-GFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004],
  2. Standard promoter [http://partsregistry.org/Part:BBa_J23101 BBa_J23101]
  3. GFP reporter devices with Weak [http://partsregistry.org/Part:BBa_J23150 BBa_J23150],
  4. Medium [http://partsregistry.org/Part:BBa_J23151 BBa_J23151] and
  5. Strong [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] promoters
      • Incubate cells in 5ml of LB broth containing the respective anitbiotics and allow them to grow overnight
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