| FIFO
|
| Construction
| Description
| Validation Date
|
   
pFliL(RBS WT)-ECFP tripart (LVA+)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.
| October 24
|
pFliL(+ RBS WT)-GFP
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.
| October 24
|
pFlgA(+ RBS WT) - YFP Tripart (LVA+)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag.
| August 22
|
pFlgA(+ RBS WT) - YFP Tripart (LVA-)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag.
| August 22
|
pFlgA(+ RBS WT)-YFP tripart (AAV+)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag.
| October 24
|
pFlgA(+ RBS WT)-GFP
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP.
| October 24
|
pFlhB(+ RBS WT)-mRFP tripart (+LVA)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag.
| October 24
|
pFlhB(+ RBS WT)-GFP
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP.
| October 26
|
| OSCILLATION
|
pFliL(+ RBS WT)-EnvZ*
|
| October 26
|
 
pFliL(+ RBS WT)-EnvZ*-GFP generator
|
| October 29
|
pFlhB(+ RBS WT)-EnvZ*
|
| October 24
|
pFlhB(+ RBS WT)-EnvZ*-GFP generator
|
| October 29
|
araC/pBad-EnvZ*
|
| October 27
|
| SYNCHRONISATION
|
pLas - gfp Tripart
|
| August 1
|
pLas - ECFP
|
| August 1
|
strongest promoter-rbs-lasR-dble ter
|
| August 20
|
Strong promoter-rbs-lasR-dble ter
|
| August 20
|
Strongest RBS (1)- LasR activator (+LVA)
|
| August 13
|
rbs-lasR + dbl ter
|
| August 19
|
lasR activator + LVA - Double Terminator
|
| August 1
|
RBS lasI - GFP tripart
|
| August 13
|
RBS-lasI - ECFP
|
| August 13
|
| NEW GENE AND PROMOTER BIOBRICKS
|
pFliA
|
| October 04
|
pFliL (+RBS WT)
|
| October 10
|
pFlgA (+RBS WT)
|
| October 10
|
pFlgB (+RBS WT)
|
| October 10
|
pFlhB (+RBS WT)
|
| October 10
|
EnvZ*
|
| September 04
|
FliA
|
| September 04
|
FlhDC
|
| August 13
|
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