FIFO
|
Construction
| Description
| Validation Date
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon reporter.png](/wiki/images/a/aa/Icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) pFliL(RBS WT)-ECFP tripart (LVA+)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.
| October 24
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon reporter.png](/wiki/images/a/aa/Icon_reporter.png) pFliL(+ RBS WT)-GFP
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.
| October 24
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon reporter.png](/wiki/images/a/aa/Icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) pFlgA(+ RBS WT) - YFP Tripart (LVA+)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag.
| August 22
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon reporter.png](/wiki/images/a/aa/Icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) pFlgA(+ RBS WT) - YFP Tripart (LVA-)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag.
| August 22
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon reporter.png](/wiki/images/a/aa/Icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) pFlgA(+ RBS WT)-YFP tripart (AAV+)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag.
| October 24
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon reporter.png](/wiki/images/a/aa/Icon_reporter.png) pFlgA(+ RBS WT)-GFP
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP.
| October 24
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon reporter.png](/wiki/images/a/aa/Icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) pFlhB(+ RBS WT)-mRFP tripart (+LVA)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag.
| October 24
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon reporter.png](/wiki/images/a/aa/Icon_reporter.png) pFlhB(+ RBS WT)-GFP
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP.
| October 26
|
OSCILLATION
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) pFliL(+ RBS WT)-EnvZ*
| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.
| October 26
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) ![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Part icon reporter.png](/wiki/images/a/a3/Part_icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) pFliL(+ RBS WT)-EnvZ*-GFP generator
| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.
| October 29
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) pFlhB(+ RBS WT)-EnvZ*
| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.
| October 24
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) ![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Part icon reporter.png](/wiki/images/a/a3/Part_icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) pFlhB(+ RBS WT)-EnvZ*-GFP generator
| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.
| October 29
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) araC/pBad-EnvZ*
| A construction made for characterizing the repression power of the mutated EnvZ on our system
| October 27
|
SYNCHRONISATION
|
![Part icon regulatory.png](/wiki/images/1/19/Part_icon_regulatory.png) ![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Part icon reporter.png](/wiki/images/a/a3/Part_icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) pLas - gfp Tripart
| Construction for characterization of the pLas promoter
| August 1
|
![Part icon regulatory.png](/wiki/images/1/19/Part_icon_regulatory.png) ![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Part icon reporter.png](/wiki/images/a/a3/Part_icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) pLas - ECFP
| The same as before but with another fluorophore
| August 1
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) strongest promoter-rbs-lasR-dble ter
| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL
| August 20
|
![Icon regulatory.png](/wiki/images/5/57/Icon_regulatory.png) ![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) Strong promoter-rbs-lasR-dble ter
| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.
| August 20
|
![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) Strongest RBS (1)- LasR activator (+LVA)
| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL
| August 13
|
![Icon rbs.png](/wiki/images/0/08/Icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) rbs-lasR + dbl ter
| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL
| August 19
|
![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) lasR activator + LVA - Double Terminator
| Intermediary construction
| August 1
|
![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) ![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Part icon reporter.png](/wiki/images/a/a3/Part_icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) RBS lasI - GFP tripart
| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.
| August 13
|
![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) ![Part icon rbs.png](/wiki/images/f/fe/Part_icon_rbs.png) ![Part icon reporter.png](/wiki/images/a/a3/Part_icon_reporter.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) ![Part icon terminator.png](/wiki/images/1/19/Part_icon_terminator.png) RBS-lasI - ECFP
| The same as the previous one but with another fluorophore.
| August 13
|
NEW GENE AND PROMOTER BIOBRICKS
|
![Part icon regulatory.png](/wiki/images/1/19/Part_icon_regulatory.png) pFliA
| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.
| October 04
|
![Part icon regulatory.png](/wiki/images/1/19/Part_icon_regulatory.png) pFliL (+RBS WT)
|
| October 10
|
![Part icon regulatory.png](/wiki/images/1/19/Part_icon_regulatory.png) pFlgA (+RBS WT)
|
| October 10
|
![Part icon regulatory.png](/wiki/images/1/19/Part_icon_regulatory.png) pFlgB (+RBS WT)
|
| October 10
|
![Part icon regulatory.png](/wiki/images/1/19/Part_icon_regulatory.png) pFlhB (+RBS WT)
|
| October 10
|
![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) EnvZ*
|
| September 04
|
![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) FliA
|
| September 04
|
![Icon coding.png](/wiki/images/b/bf/Icon_coding.png) FlhDC
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| August 13
|