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Team:UNIPV-Pavia/Notebook/Week7
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Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|---|---|---|---|---|---|
| Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
Week 7: 06/30/08 - 06/5/08
06/30/08
- We received sequencing results for BBa_B0030-BBa_C0061 (4th colony and 7th colony): sequences were correct! We decided to keep the 4th colony.
- Colony PCR for BBa_J23100-BBa_E0240 and BBa_B0030-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations.
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- We infected 9 ml LB + Amp with 30 µl of:
| BBa_C0012 | BBa_B1006 | BBa_R0051-BBa_B0030 | BBa_B0030-BBa_C0061 (4) |
| BBa_C0062 | BBa_J23100-BBa_E0240 (1) | BBa_B0030-BBa_C0078 (8) | BBa_J23100-BBa_B0030 |
| BBa_C0040 | BBa_I15010 |
- Tomorrow we will be ready to perform 5 ligations!
07/1/08
