Alberta NINT/15 July 2008
From 2008.igem.org
lab work (SD):
Since the double-plasmid cell cultures failed to grow in LB+amp50+kan35 broth, perhaps the colonies grown on LB+amp50+kan35 plates were not selected for kan resistance (ie. kan was not present on these plates). New LB+amp50+kan35 plates were made and the transformation was redone. K102022 (LB+amp50) K102032 (LB+amp50) K102032+K102011 (LB+amp50+kan35) K102032+K102034 (LB+amp50+kan35) K102032+K102035 (LB+amp50+kan35) K102032+K102036 (LB+amp50+kan35)