Virginia/18 July 2008

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Overnight

  • plates A11() A12 and A13
  • The screaning plasmid grew in overnight broth but we could not induce it to fluoresce
  • Overnight broths of BP2, BP3, PSB1A10, psb1A10 with arabinose, E0240 (3x) (needs promoter), A6(PRE BP1) with IPTG(lactose)

Goals

  • Miniprep the cells that grew in the broths
  • SDS PAGE the PRE BP1 to see if the new pathway is being expressed
  • Overnight broth of RE-BP2 and RE-BP3
  • Digest and gel miniprepped DNA from today to verify what we have
  • Try to express fluorescence by digesting and ligating PSB1a10 to
    • remove cell death gene and grow in dh5alpha cells
    • add a promoter between restriction sites to prove it can atleast fluoresce green
  • Digest and ligate E0240 (3x) with the promoter R0040 to express green


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