Wisconsin: Lignin Project/10 July 2008

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Team Sorbitol

Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.

Started the following cultures:

DH5a-PBAD30 : to harvest the plasmid to clone out the srl operon

DH5a-pBAD30-srlD: from colony 2 to freeze down for back up

DH5a-pBAD18 - to insert srld and the operon into.


MG1655, JW3890, RL257 - all to make chemically competent