Plasmid for promoter Amplification & Measurement
Those plasmids are very useful vectors to amplify promoters and to measure their forces using Standard Promoter Units.
The principle is easy to understand.
The plasmid contains the BioBrick restriction sites (EcoRI, XbaI, SpeI and PstI) followed by the standard GFP Tripart (E0240).
Instead of using a big biobrick containing Promoter, RBS, GFP and Terminators, the Biobrick used for the test is only the Promoter.
This plasmid can be used to amplify a promoter because only the promoter is in the biobrick.
Oligonucleotides design
Creation of 3 oligonucleotides (O140, O141, O142) in order to create two particular plasmids.
Necessary stuff
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