Essential publications

Project essentials

Caroline M. Ajo-Franklin et al. Rational design of memory in eukaryotic cells. (2007) PMID 17875664
Attila Becskei et al. Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion. (2001) PMID 11350942
Christina B. Tyson et al. Dependency of size of Saccharomyces cerevisiae cells on growth rate. (1978) PMID 374379
Nathan C. Shaner et al. A guide to choosing fluorescent proteins. (2005) PMID 16299475

Gudrun Bjornsdottir and Lawrence C. Myers. Minimal components of the RNA polymerase II transcription apparatus determine the consensus TATA box. (2008) PMID 18385157
Thomas Masek et al. Hepatitis C virus internal ribosome entry site initiates protein synthesis at the authentic initiation codon in yeast. (2007) PMID 17554033
Wei Zhouet et al. Isolation and identification of short nucleotide sequences that affect translation initiation in Saccharomyces cerevisiae. (2002) PMID 12679518

Gerald Thiel et al. How mammalian transcriptional repressors work. (2004) PMID 15233782
Gemma Bellí et al. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast. (1998) PMID 9461451
Jae-Ryeon Ryu et al. Cell-type specificity of short-range transcriptional repressors. (2001) PMID 11687630
Tania M. Malavé and Sharon Y.R. Dent. Transcriptional repression by Tup1-Ssn6. (2006) PMID 16936817

M. Madan Babu and Sarah A. Teichmann. Functional determinants of transcription factors in Escherichia coli: protein families and binding sites. (2003) PMID 12547514
Christian Berens and Wolfgang Hillen. Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes. (2003) PMID 12869186
A. Wissmann et al. Amino acids determining operator binding specificity in the helix-turn-helix motif of Tn10 Tet repressor. (1991) PMID 1756721
Hillary C.M. Nelson and Robert T. Sauer. Lambda repressor mutations that increase the affinity and specificity of operator binding. (1985) PMID 3161621
Nicholas Benson et al. Mutant lambda repressors with increased operator affinities reveal new, specific protein-DNA contacts. (1992) PMID 1531047
P. Oertel-Buchheit et al. Isolation and characterization of LexA mutant repressors with enhanced DNA binding affinity. (1992) PMID 1602473

Emily R. Hildebrandt and M. Andrew Hoyt. Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. (2001) PMID 11694576
Janet L. Burton and Mark Solomon. D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p. (2001) PMID 11562348
Sofie R. Salama et al. G1 cyclin degradation: the PEST motif of yeast Cln2 is necessary, but not sufficient, for rapid protein turnover. (1994) PMID 7969135
Carolina Mateus and Simon V. Avery. Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. (2000) PMID 11015728
Catherine Berset et al. Transferable domain in the G(1) cyclin Cln2 sufficient to switch degradation of Sic1 from the E3 ubiquitin ligase SCF(Cdc4) to SCF(Grr1). (2002) PMID 12052857
Matthew D. Petroski and Raymond J. Deshaies. Redundant degrons ensure the rapid destruction of Sic1 at the G1/S transition of the budding yeast cell cycle. (2003) PMID 12963827
Alexander Varshavsky. The N-end rule: functions, mysteries, uses. (1996) PMID 8901547

Gabriel Schlenstedt et al. Reconstitution of nuclear protein transport with semi-intact yeast cells. (1993) PMID 8227140
Julia M. Sidorova et al. Cell cycle-regulated phosphorylation of Swi6 controls its nuclear localization. (1995) PMID 8590795
Michelle T. Harreman et al. Regulation of nuclear import by phosphorylation adjacent to nuclear localization signals. (2004) PMID 14998990

Sung Yun Kim et al. In vivo determination of substrate specificity of hepatitis C virus NS3 protease: genetic assay for site-specific proteolysis. (2000) PMID 10933854
Hara Kang et al. An improved strategy for a genetic assay for site-specific proteolysis. (2001) PMID 11355710
Sung Yun Kim et al. Detection of site-specific proteolysis in secretory pathways. (2002) PMID 12163035

T. Chinnappan Santiago et al. The relationship between mRNA stability and length in Saccharomyces cerevisiae. (1986) PMID 3537957
Reut Shalgi et al. A catalog of stability-associated sequence elements in 3'UTRs of yeast mRNAs. (2005) PMID 16207357
Tina Gill et al. RNase MRP cleaves the CLB2 mRNA to promote cell cycle progression: novel method of mRNA degradation. (2003) PMID 14729943
Bernard J. de la Cruz et al. The role of the 5' untranslated region (UTR) in glucose-dependent mRNA decay. (2002) PMID 12112242
Benoit Pierrat et al. The 5' untranslated region of the PPR1 regulatory gene dictates rapid mRNA decay in yeast. (1993) PMID 8370540

Tom Knight. Idempotent Vector Design for Standard Assembly of Biobricks (2003) [http://web.mit.edu/synbio/release/docs/biobricks.pdf]
Ira E. Philips and Pamels A. Silver. A New Biobrick Assembly Strategy Designed for Facile Protein Engineering (2006) [http://dspace.mit.edu/bitstream/handle/1721.1/32535/PhillipsSilverFusion.pdf?sequence=1]

Timothy S. Gardner et al. Construction of a genetic toggle switch in Escherichia coli. (2000) PMID 10659857
Beat P. Kramer and Martin Fussenegger. Hysteresis in a synthetic mammalian gene network. (2005) PMID 15972812