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Edinburgh/15 July 2008
From 2008.igem.org
Week 5
Tuesday 15 July 08
- PCR P15: fusion PCR of rbs+appY using L15 as template. Extension 20s, annealing 58°C, same primer set as before. Gel 13 shows that it worked OK.
- Gel 13 also shows digests of M31 to M42 from yesterday.
- Transformation of JM109 with ligations L13 and L15 (glgC mutation and rbs+dxs cloning). Plated to plates 39 to 41.
