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Edinburgh/8 August 2008
From 2008.igem.org
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Week 8
<title>Edinburgh iGem 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07'
var tabdropdown={
- disappeardelay: 200, //set delay in miliseconds before menu disappears onmouseout
disablemenuclick: false, //when user clicks on a menu item with a drop down menu, disable menu item's link? enableiframeshim: 1, //1 or 0, for true or false
//No need to edit beyond here//////////////////////// dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null, currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
getposOffset:function(what, offsettype){ var totaloffset=(offsettype=="left")? what.offsetLeft : what.offsetTop; var parentEl=what.offsetParent; while (parentEl!=null){ totaloffset=(offsettype=="left")? totaloffset+parentEl.offsetLeft : totaloffset+parentEl.offsetTop; parentEl=parentEl.offsetParent; } return totaloffset; },
showhide:function(obj, e, obj2){ //obj refers to drop down menu, obj2 refers to tab menu item mouse is currently over if (this.ie || this.firefox) this.dropmenuobj.style.left=this.dropmenuobj.style.top="-500px" if (e.type=="click" && obj.visibility==hidden || e.type=="mouseover"){ if (obj2.parentNode.className.indexOf("default")==-1) //if tab isn't a default selected one obj2.parentNode.className="selected" obj.visibility="visible" } else if (e.type=="click") obj.visibility="hidden" },
iecompattest:function(){ return (document.compatMode && document.compatMode!="BackCompat")? document.documentElement : document.body },
clearbrowseredge:function(obj, whichedge){ var edgeoffset=0 if (whichedge=="rightedge"){ var windowedge=this.ie && !window.opera? this.standardbody.scrollLeft+this.standardbody.clientWidth-15 : window.pageXOffset+window.innerWidth-15 this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetWidth if (windowedge-this.dropmenuobj.x < this.dropmenuobj.contentmeasure) //move menu to the left? edgeoffset=this.dropmenuobj.contentmeasure-obj.offsetWidth } else{ var topedge=this.ie && !window.opera? this.standardbody.scrollTop : window.pageYOffset var windowedge=this.ie && !window.opera? this.standardbody.scrollTop+this.standardbody.clientHeight-15 : window.pageYOffset+window.innerHeight-18 this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetHeight if (windowedge-this.dropmenuobj.y < this.dropmenuobj.contentmeasure){ //move up? edgeoffset=this.dropmenuobj.contentmeasure+obj.offsetHeight if ((this.dropmenuobj.y-topedge)<this.dropmenuobj.contentmeasure) //up no good either? edgeoffset=this.dropmenuobj.y+obj.offsetHeight-topedge } this.dropmenuobj.firstlink.style.borderTopWidth=(edgeoffset==0)? 0 : "1px" //Add 1px top border to menu if dropping up } return edgeoffset },
dropit:function(obj, e, dropmenuID){ if (this.dropmenuobj!=null){ //hide previous menu this.dropmenuobj.style.visibility="hidden" //hide menu if (this.previousmenuitem!=null && this.previousmenuitem!=obj){ if (this.previousmenuitem.parentNode.className.indexOf("default")==-1) //If the tab isn't a default selected one this.previousmenuitem.parentNode.className="" } } this.clearhidemenu() if (this.ie||this.firefox){ obj.onmouseout=function(){tabdropdown.delayhidemenu(obj)} obj.onclick=function(){return !tabdropdown.disablemenuclick} //disable main menu item link onclick? this.dropmenuobj=document.getElementById(dropmenuID) this.dropmenuobj.onmouseover=function(){tabdropdown.clearhidemenu()} this.dropmenuobj.onmouseout=function(e){tabdropdown.dynamichide(e, obj)} this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)} this.showhide(this.dropmenuobj.style, e, obj) this.dropmenuobj.x=this.getposOffset(obj, "left") this.dropmenuobj.y=this.getposOffset(obj, "top") this.dropmenuobj.style.left=this.dropmenuobj.x-this.clearbrowseredge(obj, "rightedge")+"px" this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px" this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item) this.positionshim() //call iframe shim function } },
contains_firefox:function(a, b) { while (b.parentNode) if ((b = b.parentNode) == a) return true; return false; },
dynamichide:function(e, obj2){ //obj2 refers to tab menu item mouse is currently over var evtobj=window.event? window.event : e if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement)) this.delayhidemenu(obj2) else if (this.firefox&&e.currentTarget!= evtobj.relatedTarget&& !this.contains_firefox(evtobj.currentTarget, evtobj.relatedTarget)) this.delayhidemenu(obj2) },
delayhidemenu:function(obj2){ this.delayhide=setTimeout(function(){tabdropdown.dropmenuobj.style.visibility='hidden'; if (obj2.parentNode.className.indexOf('default')==-1) obj2.parentNode.className=},this.disappeardelay) //hide menu },
clearhidemenu:function(){ if (this.delayhide!="undefined") clearTimeout(this.delayhide) },
positionshim:function(){ //display iframe shim function if (this.enableiframeshim && typeof this.shimobject!="undefined"){ if (this.dropmenuobj.style.visibility=="visible"){ this.shimobject.style.width=this.dropmenuobj.offsetWidth+"px" this.shimobject.style.height=this.dropmenuobj.offsetHeight+"px" this.shimobject.style.left=this.dropmenuobj.style.left this.shimobject.style.top=this.dropmenuobj.style.top } this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none" } },
hideshim:function(){ if (this.enableiframeshim && typeof this.shimobject!="undefined") this.shimobject.style.display='none' },
isSelected:function(menuurl){ var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "") return (tabdropdown.currentpageurl==menuurl) },
init:function(menuid, dselected){ this.standardbody=(document.compatMode=="CSS1Compat")? document.documentElement : document.body //create reference to common "body" across doctypes var menuitems=document.getElementById(menuid).getElementsByTagName("a") for (var i=0; i<menuitems.length; i++){ if (menuitems[i].getAttribute("rel")){ var relvalue=menuitems[i].getAttribute("rel") document.getElementById(relvalue).firstlink=document.getElementById(relvalue).getElementsByTagName("a")[0] menuitems[i].onmouseover=function(e){ var event=typeof e!="undefined"? e : window.event tabdropdown.dropit(this, event, this.getAttribute("rel")) } } if (dselected=="auto" && typeof setalready=="undefined" && this.isSelected(menuitems[i].href)){ menuitems[i].parentNode.className+=" selected default" var setalready=true } else if (parseInt(dselected)==i) menuitems[i].parentNode.className+=" selected default" } }
} </script> <style> .ddcolortabs{ padding: 0; width: 100%; background: transparent; voice-family: "\"}\""; voice-family: inherit; }
.ddcolortabs ul{ font: normal 11px Arial, Verdana, sans-serif; margin:0; padding:0; list-style:none; }
.ddcolortabs li{ display:inline; margin:0 2px 0 0; padding:0; text-transform:uppercase; }
.ddcolortabs a{
float:left;
color: white;
background: black url(media/color_tabs_left.gif) no-repeat left top;
margin:0 2px 0 0;
padding:0 0 1px 3px;
text-decoration:none;
letter-spacing: 1px;
}
.ddcolortabs a span{ float:left; display:block; background: transparent url(media/color_tabs_right.gif) no-repeat right top; padding: 4px 8px 2px 7px; }
.ddcolortabs a span{ float:none; }
.ddcolortabs a:hover{ background-color: #591f20; }
.ddcolortabs a:hover span{ background-color: #591f20; }
.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/ background-color: #591f20; }
.ddcolortabsline{ clear: both; padding: 0; width: 100%; height: 8px; line-height: 8px; background: black; border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/ }
/* ######### Style for Drop Down Menu ######### */
.dropmenudiv_a{ position:absolute; top: 0; border: 1px solid black; /*THEME CHANGE HERE*/ border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/ border-bottom-width: 0; font:normal 12px Arial; line-height:18px; z-index:100; background-color: white; width: 200px; visibility: hidden; }
.dropmenudiv_a a{
width: auto;
display: block;
text-indent: 5px;
border-top: 0 solid #678b3f;
border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
padding: 2px 0;
text-decoration: none;
color: black;
}
- html .dropmenudiv_a a{ /*IE only hack*/
width: 100%; }
.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/ background-color: #8a3c3d; color: white; } </style> </head>
<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
- <a href="https://2008.igem.org/Team:Edinburgh" title="Home">Home</a>
- <a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a">The Project</a>
- <a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" >The Team</a>
- <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a">BioBrick Parts</a>
- <a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a">Modelling</a>
- <a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook">Notebook</a>
<script type="text/javascript"> //SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"]) tabdropdown.init("colortab", 3) </script> </html>
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Lab Notebook
Week 8
Friday 8 August 08
- Plate 94 (BABEL2+glgC-mut1,2,3) has white cultures, so can be prepped for minipreps. Plate 95 (pSB1A2+glgC-mut1,2) has blue cultures. Therefore transformation of L31 (glgC-mut1,2 to Edinbrick1) has been repeated on plates 100 and 101. (HX)
- Transformation of L32 (pSB1A2+crtB+crtI) on plates 98 and 99. (HX)
- Digested pCstA (P54) and Edinbrick1 using Spe1/EcoR1. Ligated as L33 to be transformed tomorrow (OG)
- Arabidopsis isa1 and isa2 cDNAs have arrived as well as Zea mays su1, iso2 and iso3. The Arabidopsis genes are full of forbidden restriction sites, so it would be best to use the maize genes. Primers will need to be designed. (AH)
- PCRed cenA and cex with a) KOD polymerase, b) Velocity polymerase separately to make P55 (cenA by KOD), P56 (cenA by Velocity), P57 (cex by KOD) and P58 (cex by Velocity). (Yan)
- Combined rbs+dxs with rbs+crtE as L34, and rbs+dxs with rbs+lims1 as L35. L34 and L35 ligation is being allowed to occur at 16°C overnight. (Yan)
- M79 sequencing results indicate that M79 is BABEL2+crtE. (AH)
