Edinburgh/8 August 2008

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Edinburgh iGEM 2008

Plate 94 (BABEL2+glgC-mut1,2,3) has white cultures, so can be prepped for minipreps. Plate 95 (pSB1A2+glgC-mut1,2) has blue cultures. Therefore transformation of L31 (glgC-mut1,2 to Edinbrick1) has been repeated on plates 100 and 101. (HX)
Transformation of L32 (pSB1A2+crtB+crtI) on plates 98 and 99. (HX)
Digested pCstA (P54) and Edinbrick1 using Spe1/EcoR1. Ligated as L33 to be transformed tomorrow (OG)
Arabidopsis isa1 and isa2 cDNAs have arrived as well as Zea mays su1, iso2 and iso3. The Arabidopsis genes are full of forbidden restriction sites, so it would be best to use the maize genes. Primers will need to be designed. (AH)
PCRed cenA and cex with a) KOD polymerase, b) Velocity polymerase separately to make P55 (cenA by KOD), P56 (cenA by Velocity), P57 (cex by KOD) and P58 (cex by Velocity). (Yan)
Combined rbs+dxs with rbs+crtE as L34, and rbs+dxs with rbs+lims1 as L35. L34 and L35 ligation is being allowed to occur at 16°C overnight. (Yan)
M79 sequencing results indicate that M79 is BABEL2+crtE. (AH)