Edinburgh/10 August 2008

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Edinburgh iGEM 2008

 

Week 8

Sunday 10 August 08

  • Extracted C. fimi genomic DNA, followed by PCR of rrnB (P61), cenA (P62) and cex (P63) from genomic DNA. (CF)
  • Made subcultures...
    • Patches from Plate 99 (L32, crtB+crtI) to Plate 102.
    • Patches from Plate 101 (L31, glgC double mutant+pSB1A2) to Plate 103.
    • Patches from Plates 105 (pSB1A2+crtY) and 106 (pSB1A2+rbs+crtY) to Plate 108. (CF)
  • Transformed competent cells with L36 pSB1A2+crtY (Plates 104/105) and L37 pSB1A2+rbs+crtY (Plates 106/107). (CF)
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