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Team:Chiba/protocol
From 2008.igem.org
Revision as of 03:20, 22 August 2008 by
Hiroki
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Contents
1
Transformation
1.1
Zymo comp.
1.2
Electro poration
2
DNA Purification
2.1
Sigma prep
2.2
Qiaprep
2.3
Zymo cleam
3
GEL Chack
4
Digestion
5
PCR
6
Ligation
6.1
Gel extract
6.2
Dephosphorylation of DNA
6.3
Phosphorylation of DNA
6.4
Ligation
Transformation
Zymo comp.
Electro poration
DNA Purification
Sigma prep
Qiaprep
Zymo cleam
GEL Chack
Digestion
PCR
Ligation
Gel extract
Dephosphorylation of DNA
Phosphorylation of DNA
Ligation
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