Team:Hawaii/Notebook/2008-08-20
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Things we did today
Wetlab work
Name of Task
- Margaret & Krystle
- Annealed and phosphorylated:
- plac (I14032) for front ligation
- rbs 1.0 (B0034) for back ligation
- rbs 0.6 (B0030) for front ligation
- tt (B1006) for back ligation
- We decided to perform this reaction again with out gel purification and the gel was discarded.
- These annealed products can be found in the -20°C freezer in the DNA box.
- Quantification was achieved simply by adding up the amount of DNA added.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]