Team:KULeuven/28 August 2008

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• The Team
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  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
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  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

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  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

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  Output Filter InverTimer Reset Cell Death Memory

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Lab Work

Wet Lab

• The ligations with B0015 (C0012+B0015, C0060+B0015, C0040+B0015, K145001+B0015, K145151+B0015, K45015+B0015) were electroporated again, but now they were plated on an ampicillin plate.
• A glycerol stock was made of the succeeded ligations.
• The new parts arrived today. They were also plated.
• The ligations of K145001 and K145151 with pSB1A2 were MiniPrepped and then we did a PCR to test the ligation.
• The digests of R0011, R0040+J23022, C0062 and B0033 were put on gel and purified.
• Some more digests were made, but this time we cut with SpeI, XbaI and PstI. We cut B0015 as insert and C0060, K145015, C0012, C0040 were cut as vector.

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