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Team:Chiba/protocol
From 2008.igem.org
Revision as of 12:31, 30 August 2008 by
Yoshimi
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Contents
1
Transformation
1.1
Zymo comp.
1.2
Electro poration
2
DNA Purification
2.1
Sigma prep
2.2
Qiaprep
2.3
Zymo cleam
3
GEL Check
4
Digestion
5
PCR
6
Ligation
6.1
Gel extract
6.2
Dephosphorylation of DNA
6.3
Phosphorylation of DNA
6.4
Ligation
Transformation
Zymo comp.
Electro poration
DNA Purification
Sigma prep
Qiaprep
Zymo cleam
GEL Check
Digestion
PCR
Ligation
Gel extract
Dephosphorylation of DNA
Phosphorylation of DNA
Ligation
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