From 2008.igem.org

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Things we did today

Wetlab work

Construction of p+r (cont.) and re-replacement of BB-pRL1833a MCS

Grace

EtBr stained 0.8% agarose gel ran at 60V for 1.5 hours.

• Ran RE digests from last night on gel
• Extracted bands from gel
• Ligated:

• plac+rbs and C0012 vector
• J33207 and BB-pRL1383a

• Transformed into DH5α cells

• Plated BB-pRL1383a+J33207 on LB+sp₁₀₀+X-gal and LB+sp₁₀₀+sm₁₀₀+X-gal+IPTG to verify if IPTG is necessary for blue/white screening in this strain

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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