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Team:KULeuven/19 September 2008
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Lab Work
Wet Lab
We did one last measurement of the fluorescence of GFP and GFP-LVA. After that, we started the data analysis. In each FACS measurement the fluorescence of 10,000 cells was measured. So, we calculated the mean and standard deviation (SD) of the fluorescence of all the cells in each measurement. The rest of the data analysis will be done tomorrow.
We made some new digests of the following parts (half of them failed though):
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0034 B0034] -> cut with EcoR I and Spe I (succeeded).
[http://partsregistry.org/Part:BBa_K145201 K145201] -> cut with EcoR I and Spe I (succeeded).
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23078 J23078] -> cut with EcoR I and Spe I (failed).
[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23078 J23078] -> cut with EcoR I and Spe I (failed).
[http://partsregistry.org/Part:BBa_K145275 K145275] -> cut with EcoR I and Spe I (failed).
[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] -> cut with EcoR I and Xba I (succeeded).
With these digests we could start some new ligations, they will be electroporated by one of our advisors next week:
(J23116+B0034)+(C0040+B0015) = [http://partsregistry.org/Part:BBa_K145202 K145202]
(K145201)+(K145205) = [http://partsregistry.org/Part:BBa_K145280 K145280]
(K145201)+(R0040+E0240) = [http://partsregistry.org/Part:BBa_K145279 K145279]
(J23116+B0034)+(C0062+B0015) = [http://partsregistry.org/Part:BBa_K145270 K145270]
