Team:Hawaii/Notebook/2008-09-22

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Things we did today

Wetlab work

Verification of transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. One microliter of PCR reaction were loaded into each well.
Construct Colonies
nir+rbs+pilA 95
nir+rbs+GFP 10
nir+rbs+slr1+GFPf 42
rbs+slr1+GFPf 2
rbs+GFP+tt 3
slr1+GFPf+tt 2
pSB1A3 (to self, negative control) 330
no DNA 0
  • Colony PCR of transformants
  • Restreaked and inoculated for plasmid prep:
  • rbs+GFPf+tt #1, 2
  • nir+rbs+GFP #1, 4, 7
  • nir+rbs+slr1+GFPf #2, 8
  • nir+rbs+pilA #18
  • Also inoculated using pSB1A3 for 3A vector
.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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